Using Acoustic Data Repositories to Study Vocal Responses to Playback in a Neotropical Songbird

Birds may alter song structure in response to territorial challenges to convey information about aggressive intent or fighting ability. Professional and amateur ornithologists upload daily many birdsong recordings into acoustic data repositories, usually scoring whether songs were recorded in response to a conspecific playback or produced spontaneously. We analyzed recordings from these repositories to evaluate if song traits of Rufous-browed Peppershrikes (Cyclarhis gujanensis) vary between playback-elicited songs and spontaneous songs. For each recording after playback, we chose one spatially closer spontaneous recording to avoid geographic bias. Birds recorded after playback produced slightly longer songs than birds that were singing spontaneously. This result was accounted for by increases in the amount of sound and silence within a song after the playback instead of changes in the mean number or duration of elements. Playback did not alter song frequency parameters (bandwidth, minimum, mean, and maximum frequencies) or song rate. These results indicate that song duration might mediate aggressive interactions in Rufous-browed Peppershrikes. Even considering limitations such as unknown playback stimulus identity and possible pseudoreplication, acoustic data repositories give a unique yet unexplored opportunity to gather insights into the evolution of song flexibility during aggressive encounters.