一些精子蛋白,抗氧化剂,睾酮和前列腺特异性抗原的模式在哈科特港,河流州不育男性与精子细胞畸形

Aminayanate M. Aworu, A. B. Chioma, I. Elekima, H. Brown, E. Bartimaeus
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引用次数: 1

摘要

目的:不育是一个全球性的问题,影响男性和女性。这是一种具有心理、经济和医学影响的疾病,导致创伤和压力,特别是在一个非常强调生育的社会。因此,本研究旨在探讨精子细胞畸形的不育男性精浆中某些精子蛋白、抗氧化剂和前列腺特异性抗原的模式。研究设计:本研究是一项病例对照研究,旨在调查河流州哈科特港精子细胞畸形的不育男性精液参数和精子蛋白。研究地点和时间:本研究在哈科特港河河州立大学医学检验科学系进行。不过,一些实验室调查是在哈科特港哈科特港大学教学医院的化学病理科和医学微生物科进行的。该研究在2019年9月至2022年2月期间进行。方法:共有276名男性表示有兴趣参与研究,其中193名男性受试者被招募。研究对象分为正常精子男性(100)、无精子男性(40)和少精子男性(53)。根据其精子细胞形态和活跃度,进一步将其分为弱精子型(40例)、少弱精子型(48例)、畸形精子型(26例)、弱异精子型(32例)和少弱异精子型(22例)。ELISA法测定HSP70、PKA、MDA、TAC、GPX(精浆)、PSA、睾酮(血清),分光光度法测定TAC。所得结果采用GraphPad Prism和SPSS进行统计分析。结果:精子活力、TSC和正常精子形态在少精组较差,而在OAT组非常严重。精子细胞形态异常的受试者精子蛋白发生改变。精子细胞异常的不育雄性精浆中HSP70、PKA显著升高,OPN、TAC、GPX显著降低。血浆中睾酮和PSA分别显著降低和升高。结论:氧化应激在精子细胞畸形和生育中起重要作用。OAT受试者受影响最大。
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Pattern of Some Sperm Proteins, Anti-oxidants, Testosterone, and Prostate-Specific Antigen of Infertile Males with Sperm Cell Deformities in Port Harcourt, Rivers State
Aim: Infertility is a global problem affecting both males and females. It is a condition with psychological, economic, and medical implications resulting in trauma and stress, particularly in a society where there is a strong emphasis on childbearing. The study therefore aims to investigate the pattern of some sperm proteins, anti-oxidants, and prostate-specific antigen in the seminal plasma of infertile males with sperm cell deformities. Study Design: The study is a case-control study designed to investigate semen parameters and sperm proteins in infertile males with sperm cell deformities in Port Harcourt, Rivers State. Place and Duration of Study: The study was carried out in the Department of Medical Laboratory Science, Rivers State University, Port Harcourt. However, some of the Laboratory investigations were done in the Chemical Pathology Unit and Medical Microbiology Unit of the University of Port Harcourt Teaching Hospital, Port Harcourt. The Study was between the periods of September 2019 and Feb. 2022. Methodology: A total of 276 males indicated interest to participate in the study of which 193 male subjects were recruited. These subjects were grouped into normospermic males (100), azoospermic (40) and oligospermic males (53). Based on their sperm cell morphology and active motility, they were further classified into asthenozoospermic (40), oligoasthenozoospermic (48), teratozoospermic (26), asthenoteratozoospermic (32), and oligoasthenoteratozoospermic (22). HSP70, PKA, MDA, TAC, and GPX (in seminal plasma), PSA, and testosterone (in serum) were analyzed using ELISA while TAC was done using spectrophotometric methods. Results obtained were statistically analyzed using GraphPad Prism and SPSS. Results: Sperm motility, TSC, and normal sperm morphology were poor in oligospermic subjects but were very severe in OAT subjects. Sperm proteins were altered in subjects with abnormal sperm cell morphologies. HSP70 and PKA were significantly increased while OPN, TAC, and GPX were significantly decreased in the seminal plasma of infertile males with abnormal sperm cells. Testosterone and PSA in blood plasma were significantly lower and higher respectively. Conclusion: Oxidative stress played a significant role in sperm cell deformities and fertility. OAT subjects were the most affected.
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