不同浓度辣木叶纳米颗粒膏对粪肠球菌的抑菌效果

J. J. Nugroho, Fanny Ayu Elfira
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摘要

背景:根管治疗是一种旨在消除根管系统细菌的治疗方法。粪肠球菌是导致根管治疗失败的细菌之一。化学机械制备和根管冲洗不足以完全消除细菌,因此需要根管药物使其更有效。辣木叶含有单宁、类黄酮和皂苷,具有抗菌特性。目的:研究辣木叶纳米颗粒膏对粪肠杆菌的抑菌作用。方法:采用Kirby-Bauer法进行实验室后测,对照组设计。样品由浓度为1%和2.5%的辣木叶纳米颗粒、氢氧化钙和aquadest组成,各重复3次。根据细菌在MHA琼脂培养基上饱和的抑制能力确定抑菌带的直径。结果:结果表明,浓度为1%的辣木叶纳米颗粒膏体的阻垢带平均直径为8.48 mm,浓度为2.5%的辣木叶纳米颗粒膏体的阻垢带平均直径为9.17 mm,氢氧化钙的阻垢带平均直径为10.2 mm。数据处理表明,2.5%辣木叶纳米颗粒膏组与氢氧化钙组具有相同的抗菌效果。结论:辣木叶纳米颗粒膏(辣木叶)浓度为1%和2.5%对粪肠球菌的生长均有较好的抑制作用。
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Antibacterial effectiveness of moringa leaf (Moringa oleifera) nanoparticle paste with different concentration against Enterococcus faecalis
Background: Endodontic is a treatment that aims to eliminate bacteria from the root canal system. One of the bacteria that causes root canal treatment failure is Enterococcus faecalis. Chemomechanical preparation and root canal irrigation are not sufficient to eliminate bacteria completely, so root canal medication is necessary to make it more efficient. Moringa leaves contain tannins, flavonoids and saponins which have antibacterial properties. Objective: To determine whether moringa leaf nanoparticle paste had antibacterial effectiveness against the bacterium E.faecalis. Method: Experimental laboratory with posttest with control group design with Kirby-Bauer method. The sample consisted of moringa leaf nanoparticles with concentrations of 1% and 2.5%, calcium hydroxide and aquadest with each of them being repeated 3 times. The diameter of the inhibition zone was de-termined based on the inhibitory ability of the bacteria saturated on MHA agar media. Results: the result showed that the ave-rage diameter of the inhibition zone of moringa leaf nanoparticle paste with concentrations of 1% was 8.48 mm, 2.5% was 9.17 mm and calcium hydroxide was 10.2 mm. Data processing showed that the 2.5% moringa leaf nanoparticle paste group had the same antibacterial effect as the calcium hydroxide group. Conclusion: Moringa leaf nanoparticle paste (Moringa oleifera) with concentrations of 1% and 2.5% was quite effective in inhibiting the growth of E.faecalis.
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