使用非侵入性计算机显微断层扫描技术对正常和模拟骨质疏松症动物硬组织进行形态学分析的结果

A. Dolgalev, I. Rzhepakovsky, A. Danaev, V. Avanisyan, G. Shulga, A. Korobkeev
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Bone tissue of the jaw, iliac and femur, and teeth were collected from control and experimental animals. Bone tissue samples were fixed in 10% buffered formalin. X-ray microCT scanner Skyscan 1176 (BrukermicroCT, Belgium) and software Skyscan 1176 control program (10.0.0.0), Nrecon (1.7.4.2), DataViewer (1.5.6.2), CT-analyser (1.18.4.0), CTvox (3.3.0r1403) were used to scan and process materials.Results. MicroCT examination and 3D-imaging confirmed the elimination of trabeculae in the metaphyseal region of the femur in sheep with experimental osteoporosis from the centre to the periphery; in addition, 3D-analysis showed a 15.1% decrease in bone percentage, a 7.8% decrease in bone mineral density, and an increase in Tb. Sp. (trabecular separation), Tb. Pf. (trabecular pattern factor) and SMI (structure model index) by 30.2%, 20.8% and 23.6%, respectively, and a decrease in Tb.N. (trabecular number) index by 18.6%, indicating calcium washout, decreased trabecular connectivity and a transition from a lamellar to a rod-like architecture. Similar changes were found in the 3D-analysis of the jaw bone tissue. Thus, a decrease of 18.9% in mineral density was found, as well as a significant increase of 11.58 and 2.21 in the indices, particularly Tb. Pf. and SMI. 3D-analysis of iliac microtomography also indicates a simulation of osteoporosis, as evidenced by a significant increase in the main indices characterising the development of this pathology.Conclusions. 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引用次数: 1

摘要

介绍。x射线显微断层扫描是一种非破坏性的显微结构分析方法,它具有高水平的细节,并允许使用3d分析[1]来评估器官和组织的内部结构。使用这种设备的具体工作可分为体内和体外,即在麻醉下与活体实验动物(小鼠、大鼠、兔子)一起工作,或研究从动物体内分离出来的器官和组织。本研究的目的是利用计算机显微断层扫描研究正常和模拟骨质疏松绵羊骨组织的微观结构。材料和方法。我们对正常骨质疏松症和实验性骨质疏松症绵羊的不同骨骼进行了显微ct分析。取对照动物和实验动物的颌骨、髂骨、股骨和牙齿的骨组织。骨组织样本用10%的福尔马林缓冲液固定。使用x射线微ct扫描仪Skyscan 1176 (BrukermicroCT,比利时)和软件Skyscan 1176控制程序(10.0.0.0)、Nrecon(1.7.4.2)、DataViewer(1.5.6.2)、ct - analyzer(1.18.4.0)、CTvox (3.3.0r1403)对材料进行扫描和处理。显微ct检查和3d成像证实实验性骨质疏松症羊股骨干骺端从中心到周围的小梁消失;此外,3d分析显示骨率下降15.1%,骨密度下降7.8%,结核增加。Sp.(小梁分离);Pf.(小梁模式因子)和SMI(结构模式指数)分别下降了30.2%、20.8%和23.6%。(小梁数)指数下降18.6%,表明钙冲刷,小梁连通性下降,从板层状结构过渡到棒状结构。在颌骨组织的3d分析中也发现了类似的变化。矿物密度下降了18.9%,而各项指数,尤其是结核指数,分别增加了11.58和2.21。p .和SMI。髂骨显微断层扫描的3d分析也显示了骨质疏松症的模拟,表现为该病理发展的主要指标显著增加。所获得的结果不仅客观地证明了实验动物骨质疏松症的发生,而且还表明了身体的适应-代偿反应的迹象,其特征是股骨干骺端出现大的单个小梁,骨密度和骨组织面积未表现出减少。
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RESULTS of MORPHOLOGICAL ANALYSIS of ANIMAL HARD TISSUES IN NORMAL AND SIMULATED OSTEOPOROSIS USING A NON-INVASIVE COMPUTED MICROTOMOGRAPHY TECHNIQUE
Introduction. X-ray microtomography is a non-destructive method of microstructural analysis, which has a high level of detail and allows the possibility of assessing the internal architecture of organs and tissues using 3D-analysis[1]. The specifics of working with such equipment can be divided into in vivo and in vitro, i.e. working with live laboratory animals (mice, rats, rabbits) under anesthesia or studying organs and tissues separated from the animal [2].The aim of the work was to study the microstructure of sheep bone tissues in normal and simulated osteoporosis using computed microtomography.Materials and methods. We performed microCT analysis of different sheep bones in normal and experimental osteoporosis. Bone tissue of the jaw, iliac and femur, and teeth were collected from control and experimental animals. Bone tissue samples were fixed in 10% buffered formalin. X-ray microCT scanner Skyscan 1176 (BrukermicroCT, Belgium) and software Skyscan 1176 control program (10.0.0.0), Nrecon (1.7.4.2), DataViewer (1.5.6.2), CT-analyser (1.18.4.0), CTvox (3.3.0r1403) were used to scan and process materials.Results. MicroCT examination and 3D-imaging confirmed the elimination of trabeculae in the metaphyseal region of the femur in sheep with experimental osteoporosis from the centre to the periphery; in addition, 3D-analysis showed a 15.1% decrease in bone percentage, a 7.8% decrease in bone mineral density, and an increase in Tb. Sp. (trabecular separation), Tb. Pf. (trabecular pattern factor) and SMI (structure model index) by 30.2%, 20.8% and 23.6%, respectively, and a decrease in Tb.N. (trabecular number) index by 18.6%, indicating calcium washout, decreased trabecular connectivity and a transition from a lamellar to a rod-like architecture. Similar changes were found in the 3D-analysis of the jaw bone tissue. Thus, a decrease of 18.9% in mineral density was found, as well as a significant increase of 11.58 and 2.21 in the indices, particularly Tb. Pf. and SMI. 3D-analysis of iliac microtomography also indicates a simulation of osteoporosis, as evidenced by a significant increase in the main indices characterising the development of this pathology.Conclusions. The obtained results not only objectively testify to the development of osteoporosis in the experimental animals, but also indicate signs of the adaptation-compensatory reactions of the body, characterized by appearance of large single trabeculae in the metaphysis of the femur as well as by not expressed reduction of bone mineral density and bone tissue area.
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