一种新的蛋白酶可以解释盐蒿血红蛋白结构的广泛不同模型

Geoffrey W. Krissansen, Clive N.A. Trotman, Warren P. Tate
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引用次数: 14

摘要

证据表明,一种蛋白酶是负责提出的不同模型的盐水虾的血红蛋白的结构是提出的。蛋白酶是高纯度血红蛋白制剂中的一种次要污染物,它被SDS-聚丙烯酰胺凝胶系统中的SDS激活,并容易降解血红蛋白亚基。用色谱法在Con A-Sepharose 4B上去除蛋白酶,表明血红蛋白(Mr 26 000)由Mr 13 000的两个亚基组成。
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A novel protease may explain widely differing models for the structure of Artemia salina haemoglobin

Evidence that a protease is responsible for the proposal of differing models for the structure of the haemoglobin of the brine shrimp Artemia salina is presented. The protease, a minor contaminant in highly purified haemoglobin preparations, is activated by SDS of the SDS-polyacrylamide gel system and readily degrades the haemoglobin subunit. Removal of the protease by chromatography on Con A-Sepharose 4B has shown the haemoglobin (Mr 260 000) to comprise two subunits of Mr 130 000.

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