Wulan Sasmeita, R. Suwarniaty, L. Fitri, M. Rohman, S. L. Kawuryan
{"title":"循环内皮细胞和血管细胞粘附分子-1与风湿性心脏病反流病变严重程度和心力衰竭相关","authors":"Wulan Sasmeita, R. Suwarniaty, L. Fitri, M. Rohman, S. L. Kawuryan","doi":"10.5455/JEIM.020615.OR.133","DOIUrl":null,"url":null,"abstract":"Objective: This study aims to determine the correlation of vascular cell adhesion molecule-1 (VCAM-1) and circulating endothelial cells (CEC) with cardiac valves lesion severity and heart failure (HF) in rheumatic heart disease (RHD). Methods: A cross-sectional observational study using human peripheral blood samples of 36 children aged 6 to14 years old, divided into two groups: 18 RHD patients and 18 healthy controls group. The expression of CECs and VCAM-1 on CECs was investigated by using flow-cytometry method while the levels of soluble VCAM-1 (sVCAM-1) were obtained by using ELISA method. The severity of valve lesions was determined in a qualitative manner. Results: The average of CECs expression, VCAM-1 on CECs expression, and sVCAM-1 levels were significantly higher in the patient group than those of the healthy group. The expression of VCAM-1 on CECs was significantly different among mild, moderate, and severe regurgitation. Correlation analysis showed that the CECs expression had a significant correlation with HF. Moreover, correlation was found between expression of VCAM-1 on CECs and the severity of valve lesions. However, sVCAM-1 in plasma had no correlation either with HF or with valve lesion. Conclusion: The expression of VCAM-1 on CECs is associated with severe regurgitation while CECs level was correlated with HF. VCAM-1 on CECs may be considered a marker for the severity of functional regurgitation in RHD. CECs could be recommended as a measure for RHD with HF.","PeriodicalId":16091,"journal":{"name":"Journal of Experimental and Integrative Medicine","volume":"2 1","pages":"157-164"},"PeriodicalIF":0.0000,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Circulating endothelial cells and vascular cell adhesion molecule-1 correlates with severity of regurgitation lesion and heart failure in rheumatic heart disease\",\"authors\":\"Wulan Sasmeita, R. Suwarniaty, L. Fitri, M. Rohman, S. L. Kawuryan\",\"doi\":\"10.5455/JEIM.020615.OR.133\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective: This study aims to determine the correlation of vascular cell adhesion molecule-1 (VCAM-1) and circulating endothelial cells (CEC) with cardiac valves lesion severity and heart failure (HF) in rheumatic heart disease (RHD). Methods: A cross-sectional observational study using human peripheral blood samples of 36 children aged 6 to14 years old, divided into two groups: 18 RHD patients and 18 healthy controls group. The expression of CECs and VCAM-1 on CECs was investigated by using flow-cytometry method while the levels of soluble VCAM-1 (sVCAM-1) were obtained by using ELISA method. The severity of valve lesions was determined in a qualitative manner. Results: The average of CECs expression, VCAM-1 on CECs expression, and sVCAM-1 levels were significantly higher in the patient group than those of the healthy group. The expression of VCAM-1 on CECs was significantly different among mild, moderate, and severe regurgitation. Correlation analysis showed that the CECs expression had a significant correlation with HF. Moreover, correlation was found between expression of VCAM-1 on CECs and the severity of valve lesions. However, sVCAM-1 in plasma had no correlation either with HF or with valve lesion. Conclusion: The expression of VCAM-1 on CECs is associated with severe regurgitation while CECs level was correlated with HF. VCAM-1 on CECs may be considered a marker for the severity of functional regurgitation in RHD. CECs could be recommended as a measure for RHD with HF.\",\"PeriodicalId\":16091,\"journal\":{\"name\":\"Journal of Experimental and Integrative Medicine\",\"volume\":\"2 1\",\"pages\":\"157-164\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Experimental and Integrative Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5455/JEIM.020615.OR.133\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Experimental and Integrative Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/JEIM.020615.OR.133","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Circulating endothelial cells and vascular cell adhesion molecule-1 correlates with severity of regurgitation lesion and heart failure in rheumatic heart disease
Objective: This study aims to determine the correlation of vascular cell adhesion molecule-1 (VCAM-1) and circulating endothelial cells (CEC) with cardiac valves lesion severity and heart failure (HF) in rheumatic heart disease (RHD). Methods: A cross-sectional observational study using human peripheral blood samples of 36 children aged 6 to14 years old, divided into two groups: 18 RHD patients and 18 healthy controls group. The expression of CECs and VCAM-1 on CECs was investigated by using flow-cytometry method while the levels of soluble VCAM-1 (sVCAM-1) were obtained by using ELISA method. The severity of valve lesions was determined in a qualitative manner. Results: The average of CECs expression, VCAM-1 on CECs expression, and sVCAM-1 levels were significantly higher in the patient group than those of the healthy group. The expression of VCAM-1 on CECs was significantly different among mild, moderate, and severe regurgitation. Correlation analysis showed that the CECs expression had a significant correlation with HF. Moreover, correlation was found between expression of VCAM-1 on CECs and the severity of valve lesions. However, sVCAM-1 in plasma had no correlation either with HF or with valve lesion. Conclusion: The expression of VCAM-1 on CECs is associated with severe regurgitation while CECs level was correlated with HF. VCAM-1 on CECs may be considered a marker for the severity of functional regurgitation in RHD. CECs could be recommended as a measure for RHD with HF.