Antipyretic and Anti-oxidant potential of Hydroalcoholic extract of Gendarussa vulgeris

G. vulgaris Nees of the family Apocynaceae is a medium sized tree grown in semishade or no shade and is common in the Ernad and Nilambur taluks of Kerala. Various parts of this plant have been used in the treatment of ulcers, sores, inflammation, dyspepsia, healing of wounds, etc. The present study aimed at the evaluation of anti-pyretic activity of the hydroalcoholic extracts of the leaves by in vivo methods. Phytochemical screening reveals the presences of Alkaloids, Saponins, Carbohydrates, Flavonoids and Phenols. The total phenolic content was found 0.691mg/100mg of dry weight of extract, expressed as gallic acid equivalents and the total flavonoid content was found 0.847mg/100mg, expressed as Quercetin equivalents. Antioxidant activity was performed using DPPH method. The IC50 value of Hydroalcoholic extract of Gendarussa vulgaris was found to be 55.79µg/ml. Yeast induced pyrxia and anti-pyretic property of hydroalcoholic extract of leaves of Gendarussa vulgaris. The effect of hydroalcoholic extract of leaves of Gendarussa vulgaris were determined after administration at two dose levels (100 and 200 mg/kg b.w.) in yeast induced pyrxia rats. From the results, it may be concluded that hydroalcoholic extract of leaves of Gendarussa vulgaris possess significant anti-pyretic effect may be due to the effect of antioxidants and constituent present in the leaves.