芦荟对大鼠口腔黏膜创面增殖期愈合的影响

A. Primasari, Yendriwati Yendriwati, Minasari Minasari, Erlin Kristiani Buaya, Sri Ayu Winda Madani, Kartika Nabila Sembiring, Rizka Hidayati
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摘要

芦荟是一种药用植物,含有一种活性物质乙酰甘露聚糖,被认为在加速伤口愈合方面发挥作用。本研究旨在通过观察上皮增厚、上皮间隙闭合、新生血管生长和结缔组织生长,确定芦荟提取物对大鼠口腔黏膜创面愈合增殖期的影响。本研究为纯实验研究,仅采用后测对照组设计。取24只白色Wistar大鼠(n = 24),用手术刀在口腔左侧颊黏膜上切开10 mm长、2.5 mm深的切口。将大鼠分为4组,1、2组为对照组,3、4组采用浸渍法提取芦荟提取物。在显微镜下用苏木精-伊红染色对创面组织进行组织学观察,并用图像分析仪测量上皮厚度。上皮间隙宽度分析采用形态测定法,毛细血管数量手工计数。使用Image-J软件测量结缔组织厚度。分别于治疗后第7天和第14天进行,采用独立t检验和Mann-Whitney检验对数据进行分析。结果表明,芦荟提取物对第7天上皮细胞增厚无显著影响(p = 0.701),对第14天上皮细胞增厚无显著影响(p = 0.639),但对第7天上皮细胞间隙闭合(p = 0.049)、结缔组织增厚(p = 0.004)和毛细血管增加有显著影响(p = 0.049)。由此可见,芦荟提取物可能加速口腔黏膜创面增殖期上皮间隙闭合、结缔组织增厚和毛细血管增加。
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Effect of aloe vera on the proliferation phase of oral mucosal wound healing in rats
Aloe vera is known as a medicinal plant containing an active substance acemannan thought to play a role in accelerating wound healing. This study aims to determine the effect of aloe vera extract on the proliferation phase of oral mucosa wound healing in rats by looking at epithelial thickening, epithelial gaps closure, growth of new blood vessels, and connective tissue growth. This research is a pure experimental study with a post-test only control group design. On the left buccal mucosa of the mouth of 24 white Wistar rats (n = 24), an incision was made by scalpel with 10 mm length and 2.5 mm depth. The rats were divided into 4 groups: groups 1 and 2 as controls, while groups 3 and 4 were applied with aloe vera extract made by maceration technique. Wound tissue was observed histologically with Hematoxylin-Eosin staining under a microscope with an image analyzer for measuring the thickness of epithelium. The analysis of epithelial gap width used morphometry, and the number of capillaries was counted manually. Connective tissue thickness was measured with Image-J software. These were carried out on days 7 and 14 after treatment and the data were analyzed with the independent t-test and Mann-Whitney test. The results showed that aloe vera extract had no significant effect on epithelial thickening on day 7 (p = 0.701) nor on day 14 (p = 0.639), but a significant effect was shown on epithelial gaps closing (p = 0.049), connective tissue thickening (p = 0.004), and capillary increase on day 7 (p = 0.049). It could be concluded that aloe vera extract could potentially accelerate the epithelial gaps closure, connective tissue thickening, and capillary increase during the proliferation phase of the oral mucosa wound healing.
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