A Screening of Complex Probiotics for Reducing Aspirin-Induced Gastric Injury in Mice

Peptic ulcer disease includes gastric ulcer (GU) and duodenal ulcer. GU generally refers to ulcer lesions in the gastric mucosa. The main factors that cause GU are Helicobacter pylori and nonsteroidal anti-inflammatory drugs (NSAIDs). Probiotics have been studied for many years with the effects on gastrointestinal health. Therefore, we first screened complex probiotics for the effects on reducing the NSAIDs-induced GU. 8-week-old ICR male mice were divided into a normal control group, a negative control group, a positive control group (10 mg/kg Omeprazole), and 8 groups of complex lactic acid bacteria (20.4 mg/kg/day). Except for the normal control group, mice were fed with probiotics or saline for 29 days, then 10 consecutive days with addition of 500 mg/bw/day Aspirin to induce GU. On the 40th day, mice were sacrificed for GU analysis. In the analysis of GU area, ulcer index, ulcer curative ratio, and pathological score, the combination of Lactobacillus plantarum GKD7 and Pediococcus acidilactici GKA4 was the most significant improvement. Therefore, complex strains GKD7/GKA4 would be further separated as a single strain for the mechanism studying on gastric protection.