固体发酵生产的木霉属纤维素酶

P. Sonika, Ey, M. Srivastava, M. Shahid, Vipul Kumar, A. Singh, Shubham Trivedi, Y. K. Srivastava
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引用次数: 29

摘要

本研究的主要目的是对8种木霉进行固体发酵生产纤维素酶的分析。采用了麦麸、玉米芯、蔗糖、麦芽糖和滤纸等不同的碳源。在添加玉米芯的培养基上,哈兹芽孢杆菌的纤维素酶产量最高。并对分离酶的最佳pH、温度和热稳定性进行了分析。产酶的最佳pH值在4 ~ 6之间。纤维素酶生产的最佳温度范围为30-40℃。选择最佳的pH、温度和最佳的碳源对酶的生产至关重要。与其他真菌属相比,木霉属具有更大的合成纤维素酶的潜力。
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Trichoderma species Cellulases Produced by Solid State Fermentation
The main aim of this study was to analyze eight species of Trichoderma for cellulase enzyme production by solid state fermentation. Different carbon sources such as wheat bran, corn cob, sucrose, maltose and filter paper were used. Highest celluase enzyme production was achieved with T. harzianum on media supplemented with corn cob. The optimum pH, temperature and thermal stability of isolated enzymes were also analyzed. The best pH for enzyme production was found between 4-6. The optimum temperature range for cellulase production ranged between 30-40°C. Choosing the optimum pH, temperature and best carbon source are essential for the enzyme production. Compare to other fungal genera it has been found that Trichoderma spp. have the greater potential to synthesize cellulase enzyme.
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