芽孢杆菌对植物病原菌炭疽菌的抑菌作用。

Q. G. Fadhilah, I. Santoso, A. E. Maryanto, Yasman
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引用次数: 1

摘要

植物病原真菌炭疽菌是一种引起植物病害的破坏性真菌。本研究观察了3株芽孢杆菌(Bacillus sp. KRG、KRT和LDR)对炭疽病菌(Colletotrichum sp. KA)的生物防治潜力。采用条纹和倒板双重培养法进行拮抗试验,结果表明所有芽孢杆菌菌株均能抑制炭疽病菌的生长。采用条纹法对KRG芽孢杆菌、KRT芽孢杆菌和LDR芽孢杆菌进行非延迟拮抗试验,抑菌率分别为47.88%、46.39%和46.49%。在延迟拮抗试验中,由于芽孢杆菌比炭疽杆菌早1天接种,抑制作用增强。延迟试验对芽孢杆菌KRG、KRT和LDR的抑制率分别为58.72%、55.10%和61.10%。浇板法对炭疽病菌的抑制效果影响最大。菌丝炭疽菌(Colletotrichum sp.)的生长表明,KA不能被观察到或几乎100%被抑制。
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Antifungal potential from Bacillus sp. against phytopathogenic fungus Colletotrichum sp.
The phytopathogenic fungus Colletotrichum a devastating fungal that causes plant disease. In this study, we observed the potential of three bacilli strains, Bacillus sp. KRG, KRT dan LDR as biocontrol against Colletotrichum sp. KA. Antagonist test using streak and pour plate dual culture method showed that all the Bacillus strains could inhibit growth of Colletotrichum sp. KA. The inhibition percentage by non-delayed antagonist assay using streak technique from Bacillus KRG, KRT and LDR were 47.88 %, 46.39 % and 46.49 % respectively. The inhibition increased in the delayed antagonist assay because the Bacillus strains were inoculated one day prior to Colletotrichum sp. KA. The inhibition percentage by delayed assay of Bacillus sp. KRG, KRT and LDR were 58.72 %, 55.10 % and 61.10 % respectively. The pour plate method indicated most affected technique to inhibit Colletotrichum sp. KA. It was indicated by growth of hyphae Colletotrichum sp. KA could not be observed or represented almost 100 % inhibited.
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