{"title":"通过杂交提高侧耳不同种属的产量属性","authors":"Karishmi Riba, D. K. Sarmah","doi":"10.36036/mr.30.1.2021.116223","DOIUrl":null,"url":null,"abstract":"Interspecific hybridization studies were carried out between Pleurotus sajor-caju , P. sapidus and P.flabellatus for obtaining better quality strains. Out of 48 crosses, only five inter specific crosses of P. sajor-caju x P. sapidus and four inters specific crosses of P. sajor-caju x P. flabellatus were compatible. Inter specific crossing between P. sapidus and P. flabellatus failed to show any compatible reaction. The compatible crosses were tested for evaluating their growth characteristics on MEA media and the cross SC2S1 have shown significantly higher mycelial growth rate (8.89 cm) which was followed by the cross SC1S1 (8.66 cm). The obtained hybrid crosses have shown denser and regular growth with floccose, cottony and aerial mycelial texture and also showed off-white, pure white and yellowish white in colony colour. Out of nine dikaryotic strains, the cross SC2S1 ( P. sajor-caju x P. sapidus ) was the best strain among all the obtained hybrid strains and its parental strains interms of number of days required for spawn run (11.50 days), number of days required for pin head formation (15.50 days), days required for harvesting (18.50 days), total number of fruiting body (214.00), weight of the individual fruiting body (20.00 g), total yield per bag (0.95 kg) and biological efficiency (95.00%). Whereas in terms of stipe diameter, the cross SC2F2 ( P. sajor-caju x P.flabellatus ) has shown maximum stipe diameter (3.50 cm) as compared to other dikaryotic strain and its parental strain and among the dikaryotic strain the cross SC1S2 ( P. sajor-caju x P. sapidus ) have shown significantly higher stipe length (6.41 cm), which was also higher than their respected parents. Maximum cap size (6.73 cm) was recorded in the cross SC1F2 ( P. sajor-caju x P. flabellatus ) which was significantly higher than the other dikaryotic strain and its parental strain. During sensory evaluations, the product B ( P. sajor-caju x P. flabellatus ) was rated better than the global mean in overall acceptance (8.56), taste (8.90), flavour (7.90), colour (7.40) and appearance (8.13), with highest score in all the sensory parameters followed by Product A ( P. sajor-caju x P. sapidus ). While the lowest score was obtained by P. sapidus (product D) followed by P. flabellatus (product E) and P. sajorcaju (product C).","PeriodicalId":18860,"journal":{"name":"Mushroom Research","volume":"2 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Increasing the yield attributing character of different species of Pleurotus through hybridization\",\"authors\":\"Karishmi Riba, D. K. Sarmah\",\"doi\":\"10.36036/mr.30.1.2021.116223\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Interspecific hybridization studies were carried out between Pleurotus sajor-caju , P. sapidus and P.flabellatus for obtaining better quality strains. Out of 48 crosses, only five inter specific crosses of P. sajor-caju x P. sapidus and four inters specific crosses of P. sajor-caju x P. flabellatus were compatible. Inter specific crossing between P. sapidus and P. flabellatus failed to show any compatible reaction. The compatible crosses were tested for evaluating their growth characteristics on MEA media and the cross SC2S1 have shown significantly higher mycelial growth rate (8.89 cm) which was followed by the cross SC1S1 (8.66 cm). The obtained hybrid crosses have shown denser and regular growth with floccose, cottony and aerial mycelial texture and also showed off-white, pure white and yellowish white in colony colour. Out of nine dikaryotic strains, the cross SC2S1 ( P. sajor-caju x P. sapidus ) was the best strain among all the obtained hybrid strains and its parental strains interms of number of days required for spawn run (11.50 days), number of days required for pin head formation (15.50 days), days required for harvesting (18.50 days), total number of fruiting body (214.00), weight of the individual fruiting body (20.00 g), total yield per bag (0.95 kg) and biological efficiency (95.00%). Whereas in terms of stipe diameter, the cross SC2F2 ( P. sajor-caju x P.flabellatus ) has shown maximum stipe diameter (3.50 cm) as compared to other dikaryotic strain and its parental strain and among the dikaryotic strain the cross SC1S2 ( P. sajor-caju x P. sapidus ) have shown significantly higher stipe length (6.41 cm), which was also higher than their respected parents. Maximum cap size (6.73 cm) was recorded in the cross SC1F2 ( P. sajor-caju x P. flabellatus ) which was significantly higher than the other dikaryotic strain and its parental strain. During sensory evaluations, the product B ( P. sajor-caju x P. flabellatus ) was rated better than the global mean in overall acceptance (8.56), taste (8.90), flavour (7.90), colour (7.40) and appearance (8.13), with highest score in all the sensory parameters followed by Product A ( P. sajor-caju x P. sapidus ). While the lowest score was obtained by P. sapidus (product D) followed by P. flabellatus (product E) and P. sajorcaju (product C).\",\"PeriodicalId\":18860,\"journal\":{\"name\":\"Mushroom Research\",\"volume\":\"2 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mushroom Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.36036/mr.30.1.2021.116223\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mushroom Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.36036/mr.30.1.2021.116223","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
为了获得优质的侧耳菇菌株,对侧耳菇、松皮菇和侧耳菇进行了种间杂交研究。在48个杂交组合中,只有5个种间杂交和4个种间杂交是亲和的。两种间杂交均无亲和反应。在MEA培养基上对配种杂交进行生长特性评价,配种SC2S1的菌丝生长速率显著高于配种SC2S1 (8.89 cm),其次是配种SC1S1 (8.66 cm)。所获得的杂交组合生长致密、规律,菌丝体呈絮状、棉状和气生结构,菌落颜色呈灰白色、纯白色和黄白色。在9株双核菌株中,杂交菌株SC2S1 (P. sajorw -caju x P. sapidus)在产卵所需天数(11.50 d)、大头形成所需天数(15.50 d)、收获所需天数(18.50 d)、总子实体数(214.00)、单个子实体重(20.00 g)、每袋总产量(0.95 kg)和生物效率(95.00%)方面表现最好。在柱头直径方面,杂交品种SC2F2 (P. sajora -caju x P.flabellatus)的柱头直径最大,为3.50 cm;在杂交品种SC1S2 (P. sajora -caju x P. sapidus)中,杂交品种SC2F2 (P. sajora -caju x P. sapidus)的柱头长度显著高于其亲本(6.41 cm)。杂交菌株SC1F2 (P. sajori -caju x P. flabellatus)最大帽长为6.73 cm,显著高于其他双核菌株及其亲本菌株。在感官评价中,产品B(沙参加木×扁叶木)在总体接受度(8.56)、口感(8.90)、风味(7.90)、颜色(7.40)和外观(8.13)方面的得分均高于全球平均水平,在所有感官参数中得分最高,其次是产品A(沙参加木×尖叶木)。结果表明:p (D)得分最低,其次是p (E)和p (C)。
Increasing the yield attributing character of different species of Pleurotus through hybridization
Interspecific hybridization studies were carried out between Pleurotus sajor-caju , P. sapidus and P.flabellatus for obtaining better quality strains. Out of 48 crosses, only five inter specific crosses of P. sajor-caju x P. sapidus and four inters specific crosses of P. sajor-caju x P. flabellatus were compatible. Inter specific crossing between P. sapidus and P. flabellatus failed to show any compatible reaction. The compatible crosses were tested for evaluating their growth characteristics on MEA media and the cross SC2S1 have shown significantly higher mycelial growth rate (8.89 cm) which was followed by the cross SC1S1 (8.66 cm). The obtained hybrid crosses have shown denser and regular growth with floccose, cottony and aerial mycelial texture and also showed off-white, pure white and yellowish white in colony colour. Out of nine dikaryotic strains, the cross SC2S1 ( P. sajor-caju x P. sapidus ) was the best strain among all the obtained hybrid strains and its parental strains interms of number of days required for spawn run (11.50 days), number of days required for pin head formation (15.50 days), days required for harvesting (18.50 days), total number of fruiting body (214.00), weight of the individual fruiting body (20.00 g), total yield per bag (0.95 kg) and biological efficiency (95.00%). Whereas in terms of stipe diameter, the cross SC2F2 ( P. sajor-caju x P.flabellatus ) has shown maximum stipe diameter (3.50 cm) as compared to other dikaryotic strain and its parental strain and among the dikaryotic strain the cross SC1S2 ( P. sajor-caju x P. sapidus ) have shown significantly higher stipe length (6.41 cm), which was also higher than their respected parents. Maximum cap size (6.73 cm) was recorded in the cross SC1F2 ( P. sajor-caju x P. flabellatus ) which was significantly higher than the other dikaryotic strain and its parental strain. During sensory evaluations, the product B ( P. sajor-caju x P. flabellatus ) was rated better than the global mean in overall acceptance (8.56), taste (8.90), flavour (7.90), colour (7.40) and appearance (8.13), with highest score in all the sensory parameters followed by Product A ( P. sajor-caju x P. sapidus ). While the lowest score was obtained by P. sapidus (product D) followed by P. flabellatus (product E) and P. sajorcaju (product C).