多焦点多光子显微镜:一种快速有效的三维荧光成像工具

Martin Straub, Stefan W Hell
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引用次数: 63

摘要

多焦点多光子显微镜(MMM)是一种高效且技术简单的产生多光子荧光图像的方法。具有高轴向分辨率的共聚焦和多光子扫描显微镜,MMM也实现了高速的三维显微镜。本文给出了快速模式三维显微成像的实例,包括固定脑组织成像和活体PC12细胞成像。MMM的成像速度完全由荧光光子通量决定,因此在实际应用中,对于荧光强烈的样品,可以在几秒钟内获得30-70 μm直径的约50幅图像。MMM代表了荧光标本高速非线性光学层析成像的重大进展。
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Multifocal multiphoton microscopy: A fast and efficient tool for 3-D fluorescence imaging

Multifocal multiphoton microscopy (MMM) is an efficient and technically simple method for generating multiphoton fluorescence images. Featuring the high axial resolution of confocal and multiphoton scanning microscopes, MMM also achieves high speed in 3-D microscopy. In this paper, examples of fast-mode 3-D microscopy are given including imaging of fixed brain tissue as well as living PC12 cells. The imaging speed of MMM is solely determined by the fluorescence photon flux, so that in practice, for brightly fluorescent specimens, a whole stack of about 50 images of 30–70 μm diameter can be acquired within a few seconds. MMM represents a significant advance towards high speed nonlinear optical tomography of fluorescent specimens.

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