{"title":"玉米种子中玉米蛋白的MALDI-TOF质谱分析","authors":"R. Ştefănescu, S. Băncilă","doi":"10.1515/achi-2017-0008","DOIUrl":null,"url":null,"abstract":"Abstract We report the mass spectrometric analysis of zeins extracted from degreased maize flour with either an aqueous 70% ethanol solution or 60% acetonitrile containing 10 mM dithiothreitol. The analysis was performed on a MALDI-TOF mass spectrometer using α-cyano-4-hydroxycinnamic acid as matrix. The method allowed the detection of α-, β- and γ-zeins, but not δ-zeins and was used for the analysis of zein content in the maize inbred KWS 3381 and in commercial flour at different flour particles sizes: between 710 μm and 1.0 mm, between 250 μm and 355 μm and smaller than 100 μm.","PeriodicalId":6958,"journal":{"name":"Acta Chemica Iasi","volume":"25 5 1","pages":"73 - 86"},"PeriodicalIF":0.4000,"publicationDate":"2017-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"MALDI-TOF Mass Spectrometric Analysis of Zeins Extracted from Maize Seeds\",\"authors\":\"R. Ştefănescu, S. Băncilă\",\"doi\":\"10.1515/achi-2017-0008\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract We report the mass spectrometric analysis of zeins extracted from degreased maize flour with either an aqueous 70% ethanol solution or 60% acetonitrile containing 10 mM dithiothreitol. The analysis was performed on a MALDI-TOF mass spectrometer using α-cyano-4-hydroxycinnamic acid as matrix. The method allowed the detection of α-, β- and γ-zeins, but not δ-zeins and was used for the analysis of zein content in the maize inbred KWS 3381 and in commercial flour at different flour particles sizes: between 710 μm and 1.0 mm, between 250 μm and 355 μm and smaller than 100 μm.\",\"PeriodicalId\":6958,\"journal\":{\"name\":\"Acta Chemica Iasi\",\"volume\":\"25 5 1\",\"pages\":\"73 - 86\"},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2017-07-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Chemica Iasi\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1515/achi-2017-0008\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Chemica Iasi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/achi-2017-0008","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
MALDI-TOF Mass Spectrometric Analysis of Zeins Extracted from Maize Seeds
Abstract We report the mass spectrometric analysis of zeins extracted from degreased maize flour with either an aqueous 70% ethanol solution or 60% acetonitrile containing 10 mM dithiothreitol. The analysis was performed on a MALDI-TOF mass spectrometer using α-cyano-4-hydroxycinnamic acid as matrix. The method allowed the detection of α-, β- and γ-zeins, but not δ-zeins and was used for the analysis of zein content in the maize inbred KWS 3381 and in commercial flour at different flour particles sizes: between 710 μm and 1.0 mm, between 250 μm and 355 μm and smaller than 100 μm.
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