盐酸浸泡-多级热处理法优化鸡骨明胶提取工艺

Deva Krisna Kadarani, A. Jannah
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引用次数: 1

摘要

每年增长的家禽消费率包括鸡肉,其副产品如皮和骨头的数量显著增加。即使鸡骨头含有丰富的胶原蛋白(生产明胶的主要材料),鸡骨头也不会被适当地使用。明胶通常是由牛和猪产生的,但一些健康和宗教问题已经成功地禁止使用这两种资源。鸡骨头可以作为明胶生产的替代材料。本研究旨在探讨鸡骨作为明胶生产的替代资源,采用盐酸浸没配合多级热处理。鸡骨经过几个步骤处理,如脱脂、去污、调整大小、用盐酸(1.50、3.00、4.50和6.00% v/v)浸泡24和48小时去矿化,然后用多阶段热过程(55、65和75℃)分别进行4小时的明胶提取,蒸发、干燥和形成明胶粉末。采用傅里叶红外光谱法对A型明胶的产率、水分和灰分含量、凝胶强度、酸度水平和官能团进行了研究。样品明胶已获得至少2%至8%的产率。水分和灰分含量分别适用于4 ~ 12%和0.1 ~ 0.4%的工业规格范围。在pH值为4.40 ~ 5.44的条件下,酸调过程对酸度有影响。根据明胶标准,本研究确定用6.00%盐酸浸泡24小时对鸡骨进行明胶提取的最佳工艺。在此条件下,明胶的产率达到8%以上。凝胶强度为260.57 g,乳化稳定性为90.18%,被认为是很好的明胶。样品明胶在4.5的酸度水平相当合理。利用最佳明胶样品的振动来确认蛋白质结构,也在FTIR光谱上显示了O- h, N-H和C=O等基本成分。关键词:鸡骨,盐酸浸泡,凝胶强度,乳液稳定性,FTIR光谱
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Optimization Chicken Bones Gelatin Extraction Using Hydrochloric Acid Immersion and Multi-Stage Thermal Treatment
The growing poultry consumption rate yearly includes chicken with significantly increased amounts of by-products like skin and bones. Chicken bones are unused properly even if the bone is rich in collagen which is the primary material to produce gelatin. Gelatin usually is generated by bovine and porcine, but some health and religious issues have successfully forbid using both resources. Chicken bones could be an alternative material for gelatin production. This research aimed to investigate chicken bones as a substitute resource for gelatin manufacturing using hydrochloric acid submersion in collaboration with multi-stage thermal treatment. Chicken bones were proceeded in several steps such as degreasing, decontamination, resizing, demineralization using a hydrochloric acid immersion (1.50, 3.00, 4.50, and 6.00% v/v) for 24 and 48 hours, addition gelatin extraction using multi-stages thermal process (55, 65, and 75°C) for 4 hours each temperature subsequentially, evaporating, drying, and shaping a gelatin powder. This study produced type A gelatin which investigated yield number, moisture and ash content, gel strength, acidity level, and functional group using Fourier-transform Infrared (FTIR) spectroscopy. The sample gelatin has obtained at least 2% up to 8% yields. The moisture and ash contents were suitable to the commercial specification range, 4-12%, and 0.1-0.4%, respectively. The acid conditioning process has an impact on acidity with pH levels 4.40-5.44. Based on gelatin standards, this study declared that processing chicken bones using 6.00% hydrochloric acid submersion for 24 hours was optimal for gelatin extraction. Those optimal condition has formed gelatin with more than 8% yields. It was considered great gelatin with 260.57 g Bloom of gel strength and 90.18% of emulsion stability. Sample gelatin has a quite reasonable acidity level at 4.5. Protein structures confirmation using the vibration of the best gelatin sample has also shown essential components such as O-H, N-H, and C=O on the FTIR spectrum.Keywords: chicken bones (Gallus domesticus), hydrochloric acid immersion, gel strength, emulsion stability, FTIR spectroscopy
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