绿秋葵提取物对牙根管内粪肠球菌的抑制作用

Sri Lestari, Arini Tri Jayanti, Dwi Kartika Apriyono, Sulistiyani
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引用次数: 0

摘要

粪肠球菌是一种常见于牙齿根管的细菌,即使在治疗后也能在根管内存活。使用2.5% NaOCl作为根管冲洗材料有缺点;有毒且有刺激性,所以需要其他替代品。绿秋葵提取物含有类黄酮、生物碱、皂苷、单宁和萜类化合物,具有抗菌特性。本研究旨在探讨绿秋葵提取物对粪肠球菌的抑制作用。本研究采用后测控制组设计进行实验研究。抑制试验采用圆盘扩散法,共设5个研究组,分别为:绿秋葵提取物的NaOCl浓度分别为1.563%、3.125%、6.25%、12.5%和2.5%。数据分析采用非参数统计检验(skruskal Wallis和Mann Whitney)。绿秋葵提取物的平均抑菌带直径分别为1.563% (0 mm)、3.125% (0 mm)、6.25% (15.9 mm)、12.5% (18.03 mm)和2.5% NaOCl (24.07 mm)。Kruskal Wallis检验的显著性值为0.000 (p<0.05),表明研究组之间存在差异;Mann Whitney检验的显著性值为除绿秋葵提取物浓度为1.563%和3.125%外,其余研究组之间存在显著差异。综上所述,绿秋葵提取物在浓度为6.25%和12.5%时对粪肠球菌的生长有抑制作用。
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Inhibition of green okra (Abelmoschus esculentus) extract against Enterococcus faecalis in tooth root canals
Enterococcus faecalis is a bacterium that is often found in root canals of teeth and can survive in them even after treatment. The use of 2.5% NaOCl as a root canal irrigation material has disadvantages; toxic and irritating, so other alternatives are needed. Green okra extract contains flavonoids, alkaloids, saponins, tannins, and terpenoids which have antibacterial properties. This study aims to determine the inhibition of green okra extract against E.faecalis. It was doneexperimental laboratory research with a posttest-only control group design. The inhibition test used the disc diffusion method which consisted of 5 research groups,i.e. green okra extract with concentrations of 1.563%, 3.125%, 6.25%, 12.5%, and 2.5% NaOCl. Data were analyzed using non-pa-rametric statistical testsKruskal Wallis and Mann Whitney. The average of the inhibition zone diameter of the green okra extract concentrations were 1.563% (0 mm), 3.125% (0 mm), 6.25% (15.9 mm), 12.5% (18.03 mm), and 2.5% NaOCl (24.07 mm). The Kruskal Wallis test showed a significance value of 0.000 (p<0.05) which meant that there was a difference in the research group, followed by the Mann Whitney test which showed there were significant differences between all research groups except between green okra extract concentrations of 1.563% and 3.125%.  It was concluded that green okra extract had an inhibitory effect on the growth of E.faecalis at concentrations of 6.25% and 12.5%.
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