IoT and AI-based Intelligent Agriculture framework for Crop Prediction

Currently, Artificial Intelligence (AI) and the Internet of Things (IoT) have transformed the field of agriculture with the innovative idea of automation and intelligence. The agriculture field completely relies on the uncertainty parameter of soil, atmosphere, and water. Technological advancement in IoT and AI assist in resolving this uncertainty factor and recommend the best crops to the farmers so that they can also enhance the productivity of the crops and meet the world's large food demand smartly. In this paper, we have suggested an IoT and AI-based model which trained with 2200 records of the dataset and seven attributes in Python. The model suggests 22 different crops to farmers after collecting samples through different sensor data. We used soil, temperature, humidity, pH, and rainfall sensors. Soil sensors were used to measure the amount of N, P, and K in soil. The samples of of the SLE patients, Cell culture and treatment, Plasmid construction and transfection, Quantitative real-time PCR analysis, Enzyme-linked immunosorbent assay (ELISA), Cell viability analysis, Cell apoptosis analysis, Western blot were collected. In this research, we investigated the contribution of GAS5 in the pathogenesis of SLE. We confirmed that, compared to healthy people, the expression of GAS5 was significantly decreased in peripheral monocytes of SLE patients. Subsequently, we found that GAS5 can inhibit the proliferation and promote the apoptosis of monocytes by over-expressing or knocking down the expression of GAS5. Additionally, the expression of GAS5 was suppressed by LPS. Silencing GAS5 significantly increased the expression of a group of chemokines and cytokines, including IL-1β, IL-6 and THFα, which were induced by LPS. Furthermore, it was identified that the involvement of GAS5 in TLR4-mediated inflammatory process was through affecting the activation of the MAPK signaling pathway. In general, the decreased GAS5 expression may be a potential contributor to the elevated production of a great number of cytokines and chemokines in SLE patients. And our research suggests that GAS5 contributes a regulatory role in the pathogenesis of SLE, and may provide a potential target for therapeutic intervention.