{"title":"‐Klonierung的感染cDNS (Respiratiorisches Syncytial病毒)和第一个特性Rekombinanten","authors":"S. Prösch, W. Seidel, L. Döhner, D. Liebscher","doi":"10.1002/ABIO.370060107","DOIUrl":null,"url":null,"abstract":"Genomic RNA of the Respiratory syntical virus (RS-virus) was used as a template to synthesize calfthymus DNA-primed cDNAs. These cDNAs were subsequently cloned into the E. coli plasmid pBR 322 at the PstI-site. By hybridization 6 recombinants with virusspecific cDNAs representing 10% of the whole genome could be isolated.","PeriodicalId":7037,"journal":{"name":"Acta Biotechnologica","volume":"35 1","pages":"10-13"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Klonierung von RSV‐cDNS (Respiratiorisches Syncytial Virus) und erste Charakterisierung der Rekombinanten\",\"authors\":\"S. Prösch, W. Seidel, L. Döhner, D. Liebscher\",\"doi\":\"10.1002/ABIO.370060107\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Genomic RNA of the Respiratory syntical virus (RS-virus) was used as a template to synthesize calfthymus DNA-primed cDNAs. These cDNAs were subsequently cloned into the E. coli plasmid pBR 322 at the PstI-site. By hybridization 6 recombinants with virusspecific cDNAs representing 10% of the whole genome could be isolated.\",\"PeriodicalId\":7037,\"journal\":{\"name\":\"Acta Biotechnologica\",\"volume\":\"35 1\",\"pages\":\"10-13\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1900-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Biotechnologica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/ABIO.370060107\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Biotechnologica","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/ABIO.370060107","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Klonierung von RSV‐cDNS (Respiratiorisches Syncytial Virus) und erste Charakterisierung der Rekombinanten
Genomic RNA of the Respiratory syntical virus (RS-virus) was used as a template to synthesize calfthymus DNA-primed cDNAs. These cDNAs were subsequently cloned into the E. coli plasmid pBR 322 at the PstI-site. By hybridization 6 recombinants with virusspecific cDNAs representing 10% of the whole genome could be isolated.
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