镰形副花内生真菌:吲哚乙酸的生产

R. S. Wulandari, R. Suryantini
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引用次数: 1

摘要

镰刀副蕨内生真菌的鉴定是对内生真菌作为植物激素产生物潜力的探索。植物激素是刺激芽和根形成所必需的。在镰形假檀木材逐年减少的情况下,有必要对镰形假檀进行研究。本研究的目的是鉴定镰形假单胞菌的叶、枝、根内生真菌,并测定其IAA含量。从PDA上的叶、枝和根扦插中分离得到的分离株,通过微观和宏观形态进行了鉴定。根据标准溶液的校准曲线,用可见分光光度计计算IAA的含量。从叶、枝、根中分离得到10株真菌。但从10个菌株中,只有9个可以被鉴定出来。分别为曲霉、顶孢霉、枝孢霉、木霉1、植霉、根丝胞菌、木霉2、木霉1和木霉2。Cladosporium sp.产吲哚乙酸(IAA)含量最高,为311 ppm。IAA含量最低的是根丝胞菌(Rhizoctonia sp.),为51.97 ppm。研究结果表明,根丝胞菌和根丝胞菌在镰形假蝇幼苗中作为ppgpf剂和生物农药的能力有待进一步研究。
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Endophytic Fungi in Paraserianthes falcataria: Production of Indole Acetic Acid
Identification of endophytic fungi in Paraserianthes falcatria is the effort of the potential of endophytic fungi as phytohormone producer.  Phytohormone is needed to spur shoot and root initiation.  This study in P. falcatariais necessary when woody of P. falcataria decreases every year.  The aimed of the study were to identify endophytic fungi from leaves, twigs, and roots of P. falcataria, and determine IAA content from endophytic fungi.  Isolates that were grown from leaves, twigs and roots cuttings on PDA, were identified based on micro- and macromorphology. Determining of IAA content was counted with spectrophotometer vis based on a calibration curve from the standard solution.  The results were obtained 10 of isolates fungi from leaves, twigs, and roots.  But from 10, only nine isolates that could be identified.  They were Aspergillus sp., Acremonium sp., Cladosporium sp., Trichoderma sp. 1, Phytium sp., Rhizoctonia sp., Trichoderma sp. 2, Hormiscium sp. 1 and Hormiscium sp. 2.  Production of indole acetic acid (IAA) from Cladosporium sp. had the highest content than others (311 ppm).  The lowest IAA content (51.97 ppm) was produced by the Rhizoctonia sp.  The study can be continued to find out their abilities as PGPF agents and biopesticides of P. falcataria seedlings.
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