用于光动力激光治疗的新型光敏剂

E. Voropay, M. Samtsov, E. Zhavrid, E. Aleksandrova, I. Zhuravkin
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引用次数: 0

摘要

肿瘤光动力治疗在肿瘤实验和临床中都得到了广泛的应用。在一些疾病的治疗方面取得了很大进展。但是,现在使用的光敏剂都没有提供一些要求。例如,在生物组织透明度较低的可见范围内,几乎所有生物的消光系数都很低(1.1英尺- 1.4英尺- 1厘米- 1厘米)。我们合成了用于生物组织“治疗窗口”的染料(消光系数为1 10”-3 1“M- ' cm- ' 1”)。此外,在消光最大值(760 ~ 830nm)的光谱范围内,有可靠且操作简单的激光二极管,可作为研究光动力效率的辐照源。研究了合成染料在l O ' l O '浓度的有机溶剂和水中的光谱和光化学参数。染料在有机溶剂中主要以单体形式存在。水的输入促进了染料h聚集体的形成。在发光性质上,确定了所研究染料的单线态氧生成。估计了单线态制氧的量子产率。氧敏化被定义为由于从三态染料分子到氧的非辐射能量转移。研究了6种染料的光物理和光化学性质,并对合成氧产率的二级变化进行了研究。研究了将染料导入肿瘤细胞HeLa的方法。已观察到所研究的染料在肿瘤细胞中的积累。积累的染料光谱分析在HeLa细胞中只得到单体形式。利用光谱和偏振测量方法研究了肿瘤细胞培养基中染料的环境变化。结果表明,培养基中的染料分子与蛋白聚集在一起。对六种染料在肿瘤细胞中的光动力学效率进行了测试。其中5种光敏剂在光敏剂吸收光谱范围内表现出明显的杀伤作用。每种剂的最佳光照射剂量在36-73 J/cm '范围内已经确定。测定了单线态氧的产率与染料的光动力学性质之间的关系。[11] [qh] [j] .辐射椭圆极化的影响
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New Promising Photosensityzers for Photodynamic Laser Therapy
Photodynamic therapy of tumour is whidly spread now both in experimental and clinical oncology. Great progress in care of some diseases has been achieved. However, used now photosensityzers are not provide some requirements. For example almost anyone has low extinction coefficients (1 10’-4 lo‘ M-’ cm-’) in the visible range, where biology tissues have low transparency. We synthesized dyes for the ”therapeutic window” of biology tissues (extinction coefficients are of 1 10”-3 l o ” M-’ cm-’1. Besides, for the spectral range of extinction maxima (760-830 nm) there are reliable and simple for operation laser diodes, which can be used as irradiation sources for investigations of photodynamic efficiency. Spectral and photochemical parameters of synthesized dyes in organic solvents and water with concentrations of l O ‘ l O ’ have been investigated. Dyes in organic solvents were defined to be mainly in monomeric form. The water importing promotes the formation of H-aggregates of dyes. On the luminescence properties the singlet oxygen generation of investigated dyes has been identified. Quantum yield of the singlet oxygen generation has been estimated. The oxygen sensitizing was defined to be due to nonradiative energy transfer from triplet dye molecules to oxygen. Photophysical and photochemical properties of six dyes in vitro with the two order variation of the yield of the synglet oxygen generation have been investigated. The method of dye import into tumour cells HeLa has been developed. The accumulation of investigated dyes in tumour cells has been observed. Spectral analysis of accumulated dyes got only monomer forms in HeLa cells. Spectral and polarization measurements were used to investigate the environmental variation of dyes in culture media tumour cells. It was shown that dye molecules in culture media are aggregated with the albumen. Tests of photodynamic efficiency of six dyes in the tumour cells have been made. Five of them shown evident effect of cell killing under the irradiation in the spectral range of photosensitizer absorption. Optimal doses of optical irradiation for each agent in the range 36-73 J/cm’ have been defined. The correlation between the yield of the singlet oxygen and photodynamic properties of dyes have been determined. 11 :oo QThE1 Investigation of Above-Tlirrsliold-Ionization: Influence of an Elliptical Polarization of the Rndiation
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