Rongrong Zhang, Chunqing Xie, Yayun Zhu, Liangjun Pan, Changying Yang
{"title":"dsDNA/PGA/GCE上两种血红素蛋白的电化学阻抗识别","authors":"Rongrong Zhang, Chunqing Xie, Yayun Zhu, Liangjun Pan, Changying Yang","doi":"10.1109/ICBEB.2012.13","DOIUrl":null,"url":null,"abstract":"A DNA-modified electrode was fabricated by the way of electrode modifying, namely the polymerisation-covalently bonding, for studying the recognition of DNA to heme protein. Electrochemical Impedence Resistance (EIS) showed that electron transfer resistance increased with the adding of Hemoglobin (Hb) and Cytochrome c (Cyt c). Reaction constant of Hb and DNA is K=1.13×10<sup>5</sup> L-mol<sup>-1</sup>, and K=4.08×10<sup>5</sup> L-mol<sup>-1</sup> for Cyt c, indicating that DNA-modified electrode can well recognize Hb and Cyt c.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Electrochemical Impedance Recognition for Two Heme Proteins on dsDNA/PGA/GCE\",\"authors\":\"Rongrong Zhang, Chunqing Xie, Yayun Zhu, Liangjun Pan, Changying Yang\",\"doi\":\"10.1109/ICBEB.2012.13\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A DNA-modified electrode was fabricated by the way of electrode modifying, namely the polymerisation-covalently bonding, for studying the recognition of DNA to heme protein. Electrochemical Impedence Resistance (EIS) showed that electron transfer resistance increased with the adding of Hemoglobin (Hb) and Cytochrome c (Cyt c). Reaction constant of Hb and DNA is K=1.13×10<sup>5</sup> L-mol<sup>-1</sup>, and K=4.08×10<sup>5</sup> L-mol<sup>-1</sup> for Cyt c, indicating that DNA-modified electrode can well recognize Hb and Cyt c.\",\"PeriodicalId\":6374,\"journal\":{\"name\":\"2012 International Conference on Biomedical Engineering and Biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-05-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2012 International Conference on Biomedical Engineering and Biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/ICBEB.2012.13\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2012 International Conference on Biomedical Engineering and Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/ICBEB.2012.13","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The Electrochemical Impedance Recognition for Two Heme Proteins on dsDNA/PGA/GCE
A DNA-modified electrode was fabricated by the way of electrode modifying, namely the polymerisation-covalently bonding, for studying the recognition of DNA to heme protein. Electrochemical Impedence Resistance (EIS) showed that electron transfer resistance increased with the adding of Hemoglobin (Hb) and Cytochrome c (Cyt c). Reaction constant of Hb and DNA is K=1.13×105 L-mol-1, and K=4.08×105 L-mol-1 for Cyt c, indicating that DNA-modified electrode can well recognize Hb and Cyt c.
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