DPPH法测定白石沙不同提取物对自由基的清除作用

Purendra Singh
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摘要

生物学中抗氧化剂和自由基的研究正在引发一场医学革命,预示着疾病和健康管理的新时代。本文研究了合欢的甲醇、乙酸乙酯、乙醇和水提取物的体外抗氧化作用。Benth。采用DPPH法。乙酸乙酯提取物对DPPH自由基形成的抑制作用显著,ic50值为70.5206。乙酸乙酯提取物与抗坏血酸提取物(ic50 = 36.8582)相比,具有较强的DPPH活性。IC 50值越小,表明抗氧化活性越强。综合分析结果表明,小檗(L.)的乙酸乙酯提取物Benth。是天然抗氧化剂的可行来源,可用于功能性食品和营养保健品的开发。
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Free radical scavenging effect of various extracts of Root of Shirisha (Albizia lebbeck (L.)Benth.) by DPPH method
The study of antioxidants and free radicals in biology is producing medical revolution that promises a new age disease and health management. The present study was performed to evaluate the in vitro antioxidant effect of the methanol, ethyl acetate, hydro alcohol and water extracts of Albizia lebbeck (L.) Benth. by using DPPH method. The ethyl acetate extract exhibited significant inhibition in DPPH free radical formation with IC 50 values of 70.5206. The ethyl acetate extract showed potent activity on DPPH, which is compared to that of ascorbic acid (IC 50 = 36.8582) taken as standard. The lesser IC 50 value indicates larger antioxidant activity. The results of the present comprehensive analysis demonstrated that ethyl acetate extract of Albizia lebbeck (L.) Benth. is a viable source of nature antioxidant and might be exploited for functional foods and nutraceutical applications.
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