G. Madhuri, Siddharth Kumar Palai, P. Tripathy, S. Beura
{"title":"抗氧化剂、培养条件和传代频率对蝴蝶兰杂交品种ORIANA离体培养中酚类控制的影响","authors":"G. Madhuri, Siddharth Kumar Palai, P. Tripathy, S. Beura","doi":"10.9734/ijpss/2022/v34i230837","DOIUrl":null,"url":null,"abstract":"The present study was conducted to control phenols in the media during in vitro cultures using flower stalk explants of Phalaenopsis hybrid cultivar Oriana. MS medium + BAP (1.00 ppm) + NAA (0.50 ppm) + 3% sucrose (w/v) was used commonly for all the treatment. Results revealed that inclusion of 500 ppm citric acid in the media, rinsing the explants with citric acid 500 ppm for 20 minutes before inoculation, keeping culture in dark for 20 days and sub-culturing at 4, 6 and 8 days after inoculation were found to be significantly reducing the browning intensity (very low browning) in medium. The explants remained green even after 4th week of culture with significantly minimum percentage of blackening of cultures (10.00%), minimum percentage of dead cultures (20.00%) and maximum percentage of cultures survived (80.00%).Inclusion of 500 ppm citric acid in the media, rinsing of explant with citric acid 500 ppm for 20 minutes before inoculation, incubating the culture in dark for 20 days and subculturing at an interval of 4, 6, and 8 day after inoculation in case of flower stalk explants were observed to control phenols in the media during in vitro cultures of Phalaenopsis hybrid cultivar Oriana.","PeriodicalId":14335,"journal":{"name":"International Journal of Plant & Soil Science","volume":"25 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of Antioxidants, Culture Conditions and Frequency of Subculture to Control Phenols during in vitro Culture of Phalaenopsis Hybrid Cultivar ORIANA\",\"authors\":\"G. Madhuri, Siddharth Kumar Palai, P. Tripathy, S. Beura\",\"doi\":\"10.9734/ijpss/2022/v34i230837\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The present study was conducted to control phenols in the media during in vitro cultures using flower stalk explants of Phalaenopsis hybrid cultivar Oriana. MS medium + BAP (1.00 ppm) + NAA (0.50 ppm) + 3% sucrose (w/v) was used commonly for all the treatment. Results revealed that inclusion of 500 ppm citric acid in the media, rinsing the explants with citric acid 500 ppm for 20 minutes before inoculation, keeping culture in dark for 20 days and sub-culturing at 4, 6 and 8 days after inoculation were found to be significantly reducing the browning intensity (very low browning) in medium. The explants remained green even after 4th week of culture with significantly minimum percentage of blackening of cultures (10.00%), minimum percentage of dead cultures (20.00%) and maximum percentage of cultures survived (80.00%).Inclusion of 500 ppm citric acid in the media, rinsing of explant with citric acid 500 ppm for 20 minutes before inoculation, incubating the culture in dark for 20 days and subculturing at an interval of 4, 6, and 8 day after inoculation in case of flower stalk explants were observed to control phenols in the media during in vitro cultures of Phalaenopsis hybrid cultivar Oriana.\",\"PeriodicalId\":14335,\"journal\":{\"name\":\"International Journal of Plant & Soil Science\",\"volume\":\"25 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-02-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Plant & Soil Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.9734/ijpss/2022/v34i230837\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Plant & Soil Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9734/ijpss/2022/v34i230837","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Effect of Antioxidants, Culture Conditions and Frequency of Subculture to Control Phenols during in vitro Culture of Phalaenopsis Hybrid Cultivar ORIANA
The present study was conducted to control phenols in the media during in vitro cultures using flower stalk explants of Phalaenopsis hybrid cultivar Oriana. MS medium + BAP (1.00 ppm) + NAA (0.50 ppm) + 3% sucrose (w/v) was used commonly for all the treatment. Results revealed that inclusion of 500 ppm citric acid in the media, rinsing the explants with citric acid 500 ppm for 20 minutes before inoculation, keeping culture in dark for 20 days and sub-culturing at 4, 6 and 8 days after inoculation were found to be significantly reducing the browning intensity (very low browning) in medium. The explants remained green even after 4th week of culture with significantly minimum percentage of blackening of cultures (10.00%), minimum percentage of dead cultures (20.00%) and maximum percentage of cultures survived (80.00%).Inclusion of 500 ppm citric acid in the media, rinsing of explant with citric acid 500 ppm for 20 minutes before inoculation, incubating the culture in dark for 20 days and subculturing at an interval of 4, 6, and 8 day after inoculation in case of flower stalk explants were observed to control phenols in the media during in vitro cultures of Phalaenopsis hybrid cultivar Oriana.