{"title":"在无细胞系统中杂交的谷氨酸对谷氨酸无效","authors":"Dieter Mecke, Karl Wulff, Helmut Holzer","doi":"10.1016/0926-6593(66)90016-6","DOIUrl":null,"url":null,"abstract":"<div><p>A system is described in which glutaminine synthetase (EC 6.3.1.2) from <em>Escherichia coli</em> B is irreversibly inactivated by incubation with ATP, Mg<sup>2+</sup> and gluatmine or glutamate <em>in vitro</em>. Under the conditions chose, the glutamyl transferase activity does not change significantly.</p><p>Mechanisms are discussed for the in activation of glutamine synthetase <em>in vitro</em> as well as the relationship of this process to the inactivation of the enzyme by NH<sub>4</sub><sup>+</sup> in intact cells.</p></div>","PeriodicalId":100160,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation","volume":"128 3","pages":"Pages 559-567"},"PeriodicalIF":0.0000,"publicationDate":"1966-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6593(66)90016-6","citationCount":"32","resultStr":"{\"title\":\"Metabolit-induzierte inaktivierung von glutaminsynthetase aus Escherichia coli im zellfreien system\",\"authors\":\"Dieter Mecke, Karl Wulff, Helmut Holzer\",\"doi\":\"10.1016/0926-6593(66)90016-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A system is described in which glutaminine synthetase (EC 6.3.1.2) from <em>Escherichia coli</em> B is irreversibly inactivated by incubation with ATP, Mg<sup>2+</sup> and gluatmine or glutamate <em>in vitro</em>. Under the conditions chose, the glutamyl transferase activity does not change significantly.</p><p>Mechanisms are discussed for the in activation of glutamine synthetase <em>in vitro</em> as well as the relationship of this process to the inactivation of the enzyme by NH<sub>4</sub><sup>+</sup> in intact cells.</p></div>\",\"PeriodicalId\":100160,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation\",\"volume\":\"128 3\",\"pages\":\"Pages 559-567\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1966-12-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6593(66)90016-6\",\"citationCount\":\"32\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926659366900166\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926659366900166","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Metabolit-induzierte inaktivierung von glutaminsynthetase aus Escherichia coli im zellfreien system
A system is described in which glutaminine synthetase (EC 6.3.1.2) from Escherichia coli B is irreversibly inactivated by incubation with ATP, Mg2+ and gluatmine or glutamate in vitro. Under the conditions chose, the glutamyl transferase activity does not change significantly.
Mechanisms are discussed for the in activation of glutamine synthetase in vitro as well as the relationship of this process to the inactivation of the enzyme by NH4+ in intact cells.
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