在无细胞系统中杂交的谷氨酸对谷氨酸无效

Dieter Mecke, Karl Wulff, Helmut Holzer
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引用次数: 32

摘要

本文描述了一个系统,其中来自大肠杆菌B的谷氨酰胺合成酶(EC 6.3.1.2)通过ATP、Mg2+和谷氨酰胺或谷氨酸体外孵育而不可逆地失活。在所选条件下,谷氨酰转移酶活性变化不显著。本文讨论了体外谷氨酰胺合成酶的激活机制,以及这一过程与完整细胞中NH4+使谷氨酰胺合成酶失活的关系。
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Metabolit-induzierte inaktivierung von glutaminsynthetase aus Escherichia coli im zellfreien system

A system is described in which glutaminine synthetase (EC 6.3.1.2) from Escherichia coli B is irreversibly inactivated by incubation with ATP, Mg2+ and gluatmine or glutamate in vitro. Under the conditions chose, the glutamyl transferase activity does not change significantly.

Mechanisms are discussed for the in activation of glutamine synthetase in vitro as well as the relationship of this process to the inactivation of the enzyme by NH4+ in intact cells.

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