Ryan David Pandapotan Hutahaean, D. Meles, Ratih Novita Praja, J. Rahmahani, Prima Ayu Wibawati
{"title":"Gamal叶乙醇提取物对半天然海龟巢大肠杆菌生长的影响","authors":"Ryan David Pandapotan Hutahaean, D. Meles, Ratih Novita Praja, J. Rahmahani, Prima Ayu Wibawati","doi":"10.20473/jmv.vol5.iss2.2022.225-233","DOIUrl":null,"url":null,"abstract":"The aim of this study was to identify the antibacterial activity potention of gamal leaf (Gliricidia sepium) ethanol extract against Escherichia coli growth which isolated from Olive Ridley Sea Turtle (Lepidochelys olivacea) semi-natural nest in Boom beach, Banyuwangi. The antibacterial test used disk diffusion method. Oxytetracycline 30 µg (Oxoid CT0041B) was used as the Positive Control (K+). CMC Na 0,5% was used as the Negative Control (K-). The treatment (P1, P2, P3, P4) used various concentrations of gamal leaves ethanol extract, which is 40%, 50%, 60%, and 70%. The study assigned six treatments and four replication. The obtained data were analyzed by ANOVA, then continued with the DUNCAN test if the results showed significant differences (p<0,05). The inhibition result of positive control (K+) was 24,70c ± 7.04; and the negative control was 0,00a ± 0,00. All extract concentration result different inhibition, concentration 1 (P1) was 10,41 b ± 3,10; concentration 2 (P2) 8,14 b ± 0,45; concentration 3 (P3) 8,08 b ± 0,47; and concentration 4 (P4) 8,01b ± 0,29. These results indicated that the gamal leaves ethanol extract has antibacterial activity to inhibit the growth of E. coli isolated from Olive Ridley semi-natural nest in Boom beach, Banyuwangi.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"2 1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Effect of The Gamal Leaf Ethanol Extract on The Growth of Escherichia coli Isolated from Semi Natural Sea Turtle Nest in Boom Beach, Banyuwangi\",\"authors\":\"Ryan David Pandapotan Hutahaean, D. Meles, Ratih Novita Praja, J. Rahmahani, Prima Ayu Wibawati\",\"doi\":\"10.20473/jmv.vol5.iss2.2022.225-233\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The aim of this study was to identify the antibacterial activity potention of gamal leaf (Gliricidia sepium) ethanol extract against Escherichia coli growth which isolated from Olive Ridley Sea Turtle (Lepidochelys olivacea) semi-natural nest in Boom beach, Banyuwangi. The antibacterial test used disk diffusion method. Oxytetracycline 30 µg (Oxoid CT0041B) was used as the Positive Control (K+). CMC Na 0,5% was used as the Negative Control (K-). The treatment (P1, P2, P3, P4) used various concentrations of gamal leaves ethanol extract, which is 40%, 50%, 60%, and 70%. The study assigned six treatments and four replication. The obtained data were analyzed by ANOVA, then continued with the DUNCAN test if the results showed significant differences (p<0,05). The inhibition result of positive control (K+) was 24,70c ± 7.04; and the negative control was 0,00a ± 0,00. All extract concentration result different inhibition, concentration 1 (P1) was 10,41 b ± 3,10; concentration 2 (P2) 8,14 b ± 0,45; concentration 3 (P3) 8,08 b ± 0,47; and concentration 4 (P4) 8,01b ± 0,29. These results indicated that the gamal leaves ethanol extract has antibacterial activity to inhibit the growth of E. coli isolated from Olive Ridley semi-natural nest in Boom beach, Banyuwangi.\",\"PeriodicalId\":17972,\"journal\":{\"name\":\"Jurnal Medik Veteriner\",\"volume\":\"2 1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-10-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Jurnal Medik Veteriner\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.20473/jmv.vol5.iss2.2022.225-233\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jurnal Medik Veteriner","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.20473/jmv.vol5.iss2.2022.225-233","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The Effect of The Gamal Leaf Ethanol Extract on The Growth of Escherichia coli Isolated from Semi Natural Sea Turtle Nest in Boom Beach, Banyuwangi
The aim of this study was to identify the antibacterial activity potention of gamal leaf (Gliricidia sepium) ethanol extract against Escherichia coli growth which isolated from Olive Ridley Sea Turtle (Lepidochelys olivacea) semi-natural nest in Boom beach, Banyuwangi. The antibacterial test used disk diffusion method. Oxytetracycline 30 µg (Oxoid CT0041B) was used as the Positive Control (K+). CMC Na 0,5% was used as the Negative Control (K-). The treatment (P1, P2, P3, P4) used various concentrations of gamal leaves ethanol extract, which is 40%, 50%, 60%, and 70%. The study assigned six treatments and four replication. The obtained data were analyzed by ANOVA, then continued with the DUNCAN test if the results showed significant differences (p<0,05). The inhibition result of positive control (K+) was 24,70c ± 7.04; and the negative control was 0,00a ± 0,00. All extract concentration result different inhibition, concentration 1 (P1) was 10,41 b ± 3,10; concentration 2 (P2) 8,14 b ± 0,45; concentration 3 (P3) 8,08 b ± 0,47; and concentration 4 (P4) 8,01b ± 0,29. These results indicated that the gamal leaves ethanol extract has antibacterial activity to inhibit the growth of E. coli isolated from Olive Ridley semi-natural nest in Boom beach, Banyuwangi.