Gamal叶乙醇提取物对半天然海龟巢大肠杆菌生长的影响

Ryan David Pandapotan Hutahaean, D. Meles, Ratih Novita Praja, J. Rahmahani, Prima Ayu Wibawati
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摘要

本研究旨在研究gamal叶(Gliricidia sepium)乙醇提取物对半天然榄蠵龟(Lepidochelys olivacea)巢中大肠杆菌的抑菌活性。抗菌试验采用纸片扩散法。以土霉素30µg (Oxoid CT0041B)作为阳性对照(K+)。以CMC Na 0.5%作为阴性对照(K-)。处理(P1, P2, P3, P4)使用不同浓度的甘麻叶乙醇提取物,分别为40%,50%,60%和70%。该研究分配了6个处理和4个重复。对所得数据进行方差分析,若结果有显著性差异(p< 0.05),则继续进行DUNCAN检验。阳性对照(K+)抑制结果为24,70c±7.04;阴性对照为0万±0万。各提取液浓度均有不同的抑制效果,浓度1 (P1)为10、41±3、10;浓度2 (P2) 8,14 b±0,45;浓度3 (P3) 8,08±0,47;浓度4 (P4) 8,01b±0,29。上述结果表明,gamal叶乙醇提取物具有抑菌活性,可以抑制Banyuwangi Boom beach地区Olive Ridley半天然巢中大肠杆菌的生长。
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The Effect of The Gamal Leaf Ethanol Extract on The Growth of Escherichia coli Isolated from Semi Natural Sea Turtle Nest in Boom Beach, Banyuwangi
The aim of this study was to identify the antibacterial activity potention of gamal leaf (Gliricidia sepium) ethanol extract against Escherichia coli growth which isolated from Olive Ridley Sea Turtle (Lepidochelys olivacea) semi-natural nest in Boom beach, Banyuwangi. The antibacterial test used disk diffusion method. Oxytetracycline 30 µg (Oxoid CT0041B) was used as the Positive Control (K+). CMC Na 0,5% was used as the Negative Control (K-). The treatment (P1, P2, P3, P4) used various concentrations of gamal leaves ethanol extract, which is 40%, 50%, 60%, and 70%. The study assigned six treatments and four replication. The obtained data were analyzed by ANOVA, then continued with the DUNCAN test if the results showed significant differences (p<0,05). The inhibition result of positive control (K+) was 24,70c ± 7.04; and the negative control was 0,00a ± 0,00. All extract concentration result different inhibition, concentration 1 (P1) was 10,41 b ± 3,10; concentration 2 (P2) 8,14 b ± 0,45; concentration 3 (P3) 8,08 b ± 0,47; and concentration 4 (P4) 8,01b ± 0,29. These results indicated that the gamal leaves ethanol extract has antibacterial activity to inhibit the growth of E. coli isolated from Olive Ridley semi-natural nest in Boom beach, Banyuwangi.
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