丁香假单胞菌病原菌血清分型方案的现代化

Q4 Biochemistry, Genetics and Molecular Biology Mikrobiolohichnyi zhurnal Pub Date : 2022-12-17 DOI:10.15407/microbiolj84.03.009
L. Pasichnyk, L. Butsenko
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引用次数: 0

摘要

1979年,L.T. Pastushenko和i.d ymonovych提出了假单胞菌属植物致病菌血清分型方案,该方案沿用至今。然而,由于缺乏多年来积累的所有数据,今天使用这种血清分组方案变得复杂。此外,该方案不符合假单胞菌属植物致病菌的现代分类。的目标。在自身实验结果和科学文献资料的基础上,对假单胞菌属植物致病菌的血清分型方案进行现代化的研究。方法。从乌克兰不同地区的小麦、黑麦、燕麦、烟草和各种受影响的杂草中分离出的丁香假单胞菌病原菌株,如atrofaciens、coronafaciens、tabaci菌株。用抗血清对丁香假单胞菌9个血清学组(I、II、III、IV、V、VI、VII、VIII、IX)进行凝集和沉淀反应(Ouchterlony双免疫扩散技术),研究菌株的抗原性。沉淀反应采用改进的Grasse法获得O-和oh -抗原。根据L.T. Pastushenko和i.d ymonovych于1979年建立的已知植物致病菌血清分型方案,所研究抗原的沉淀线数目与相应血清群的同源抗血清的沉淀线数目相同,证明它们属于该血清群。结果。已经证明,从不同谷物(黑麦、小麦、燕麦)和部分植物中分离的丁香假单胞菌病原菌的抗原组成不同。丁香假单胞菌菌株的抗原组成取决于其分离的寄主植物。丁香腐病病原菌的研究。从小麦中分离到的atrofaciens可分为4个血清学类群(II、IV、V、VI),从黑麦中分离到5个血清学类群(I、II、IV、V、VI),以及从小麦农藓病的分生植被中分离到的菌株。丁香晕疫病菌株的研究。来自受感染燕麦植株的冠状病毒分属于两个血清学类群(I, V)。该血清分型方案得到了syringae pv抗原特性新数据的补充。番茄细菌性斑点病(Solanum lycopersicum)的病原,被归类为血清IV群。引起烟草野火的烟粉虱菌株属于三种血清群——VII、VIII、IX的一部分,而不是像已知方案(1979年)中提出的那样属于两种血清群。结论。因此,我们在自己的研究和文献资料的基础上,对L.T. Pastushenko和i.d ymonovych开发的假单胞菌属植物致病菌的血清分型方案进行了现代化的研究。在修改后的方案中,现有的植物病原体的物种名称,该方案引入了新的信息,关于P. syringae pv血清群。从黑麦种子和植物、杂草中分离得到atrofaciens;冠状病毒-来自燕麦植物,紫丁香P.;烟草(tabaci) -来自烟草;番茄-取自受影响的番茄植株。
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Modernization of the Pseudomonas syringae Pathovars Serogrouping Scheme
In 1979, L.T. Pastushenko and I.D. Symonovych developed a scheme of serogrouping phytopathogenic bacteria of the Pseudomonas genus, which is still used now. However, today’s using this serogrouping scheme is complicated by the lack of all data accumulated over the years of its application. Moreover, the scheme does not correspond to the modern taxonomy of phytopathogenic bacteria of the Pseudomonas genus. Aim. On the basis of own experimental results and data of scientific literature, to carry out modernization of the serogrouping scheme of phytopathogenic bacteria of the Pseudomonas genus. Methods. The strains of Pseudomonas syringae pathovars such as atrofaciens, coronafaciens, tabaci, which were isolated from plants of wheat, rye, oats, tobacco, and various species of affected weeds in different regions of Ukraine have been studied in the work. Antigenic properties of bacterial strains were studied by agglutination and precipitation reactions (the Ouchterlony double immunodiffusion techniques) using antisera to P. syringae strains of nine serological groups (I, II, III, IV, V, VI, VII, VIII, IX). To carry out the precipitation reaction, O- and OH-antigens were obtained by a modified Grasse’s method. The presence of the same number of precipitation lines of the studied antigens as the number of lines with homologous antiserum of the corresponding serogroup testified to their belonging to this serogroup according to the known serogrouping scheme of phytopathogenic bacteria developed in 1979 by L.T. Pastushenko and I.D. Symonovych. Results. It has been proved that strains of P. syringae pathovars isolated from different cereals (rye, wheat, oats) and segetal vegetation differ in antigenic composition. The antigenic composition of P. syringae strains depends on the host plant from which the pathogen was isolated. Strains of the causative agent of basal glume rot P. syringae pv. atrofaciens isolated from wheat belong to four serological groups (II, IV, V, VI), from rye — to five serological groups (I, II, IV, V, VI), as well as strains of this pathogen isolated from segetal vegetation of wheat agrophytocenosis. Strains of the halo bligth P. syringae pv. coronafaciens from affected oat plants belong to two serological groups (I, V). The serogrouping scheme has been supplemented by new data on the antigenic properties of P. syringae pv. tomato, the causative agent of the bacterial speck disease of tomatoes (Solanum lycopersicum), which is classified as serogroup IV. It has been found that P. syringae pv. tabaci strains, which cause wildfire of tobacco, are part of three serogroups — VII, VIII, IX, and not of two ones, as was presented in the known scheme (1979). Conclusions. Therefore, on the basis of our own research and literature data, the serogrouping scheme of phytopathogenic bacteria of the Pseudomonas genus developed by L.T. Pastushenko and I.D. Symonovych has been modernized. In the renovated scheme, current species’ names of phytopathogens are present, this scheme introduces new information about the serogroups of P. syringae pv. atrofaciens isolated from rye seeds and plants, weeds, P. syringae pv. coronafaciens — from oat plants, P. syringae pv. tabaci — from tobacco, and P. syringae pv. tomato — from affected tomato plants.
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Mikrobiolohichnyi zhurnal
Mikrobiolohichnyi zhurnal Medicine-Microbiology (medical)
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