摩洛哥佩皮诺花叶病毒分离株的遗传特征

A. Souiri, M. Zemzami, Hayat Laatiris, S. Amzazi, M. Ennaji
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引用次数: 1

摘要

在过去的几年中,胡椒花叶病毒(PepMV)已迅速从一种新兴病毒演变为地方性病原体,造成全球番茄作物的重大损失。可靠的检测和分子表征是支持疾病控制的非常重要的工具。交叉保护也可以是一种有效的策略,但效果很大程度上取决于基因型。摩洛哥PepMV人群的遗传组成尚未确定。目前的研究旨在通过分析RNA依赖性RNA聚合酶(RdRp)、三重基因块(TGB)和外壳蛋白(CP)基因的部分核苷酸序列,对来自不同摩洛哥番茄产区的12株PepMV分离株(PepMV- ma)进行遗传表征。对12株PepMV-MA分离株的序列分析表明,它们之间存在微小的核苷酸差异,表明它们是同质群体。通过与主要基因型的比较,系统发育分析表明,摩洛哥人群与智利菌株(CH2)具有非常高的序列同源性(98%),而与欧洲菌株(EU)的同源性仅为85%。有趣的是,摩洛哥分离株显示出特定的单核苷酸多态性,其中一些导致氨基酸变化。这些突变以前从未被描述过,这表明不同的变异可能会增强侵袭性和症状。我们仔细的序列分析和基因型确定,将摩洛哥同源PepMV菌株置于CH2基因型中,将是部署有效交叉保护策略以在现场控制病原体的先决条件。
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Genetic Characterization of Pepino Mosaic Virus Isolates from Morocco
Throughout the past few years, Pepino Mosaic Virus (PepMV) has rapidly evolved from an emerging virus to endemic pathogen that causes significant losses in tomato crops worldwide. Reliable detection and molecular characterization are very important tools to support disease control. Cross-protection can also be an effective strategy, but the efficacy depends strongly on the genotype. The genetic composition of the PepMV population in Morocco has not yet been determined. The current study aims to genetically characterize twelve PepMV isolates (PepMV-MA), all from different Moroccan tomato production areas, by analyzing nucleotide sequences of a part of the RNA-dependent RNA polymerase (RdRp), Triple Gene Block (TGB) and Coat Protein (CP) genes. The sequence analysis of the twelve PepMV-MA isolates shows minor nucleotide differences between them, which implies a homogenous population. The phylogenetic analysis, based on the comparison with the major genotypes, showed that Moroccan PepMV populations share a very high sequence identity, 98%, with the Chilean strain (CH2), while the shared identity with the European strains (EU) is only 85%. Interestingly, Moroccan isolates reveal specific single nucleotide polymorphisms, some of which lead to amino acids changes. These mutations have never been described before, suggesting distinct variants that may enhance aggressiveness and symptomatology. Our careful sequence analysis and genotype determination, which placing homogenous Moroccan PepMV strains into CH2 genotype, would be a prerequisite for deploying effective cross-protection strategies for controlling the pathogen in the field.
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