PAX草药保健茶和PAX草药茶的生物活性成分及薄层色谱谱数据

Tunde Ayobami Owolabi, E. Amodu
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摘要

背景:草药的植物成分在很大程度上受到生产过程中和生产后过程中使用的质量控制系统的影响,包括这些产品的处理。薄层色谱法就是这样一种质量控制参数,它显示了各种物质之间的唯一性和均匀性,可以作为这些物质的身份。Â Pax草药保健茶(PHT)和Pax草药茶(PDT)是多种草药,PHT用于一般健康的滋补品,而PDT用于糖尿病的管理和治疗。本研究评估了不同的植物成分,并建立了PHT和PDT的薄层色谱(TLC)指纹图谱,作为生产过程中的质量控制检查,以确保生产后的一致性和市场独特性。材料和方法:采用标准方法进行定性植物化学和色谱分析。结果:植物筛选结果显示,PHT中存在生物碱、黄酮类化合物、单宁、萜类化合物、还原糖、类固醇和心糖苷,而PDT中存在皂苷、单宁、类固醇、还原糖、黄酮类化合物和萜类化合物。正己烷:乙酸乙酯(3:2)显影后的PHT在365 nm紫外光下有4个明显的荧光成分,在可见光下喷20%甲醇硫酸时有3个斑点,而正己烷:乙酸乙酯:甲醇(2.5:2:0.5)显影后的PDT在365 nm有3个荧光成分,硫酸处理后有4个荧光成分。结论:本研究通过中药的生物活性成分和薄层色谱图谱设计了中药的鉴别卡,可用于评价中药的质量和一致性。一个一个
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Bioactive composition and TLC profile data on PAX herbal health tea and PAX herbal diatea
Background: The phytoconstituents of herbal drugs are largely influenced by the quality control system used during and post-production processes including the handling of such products. Thin Layer Chromatography is one of such quality control parameters that demonstrate uniqueness and uniformity between various substances, serving as an identity for such substances.  Pax Herbal Health tea (PHT) and Pax Herbal Diatea (PDT) are polyherbal drugs, PHT is used as a tonic for general wellness, while, PDT is used in the management and treatment of diabetes. This study evaluated the different phytoconstituents present and developed Thin-layer chromatography (TLC) fingerprint profiles for PHT and PDT to serve as quality control checks during the production for consistency and market uniqueness after production.Material and Methods: Qualitative phytochemical and chromatographic analyses were carried out using standard methods.Results: The phyto-screening revealed the presence of Alkaloid, Flavonoid, Tanin, Terpenoids, Reducing sugar, Steroid, and Cardiac glycoside in PHT while, Saponin, Tanin, Steroids, Reducing sugar, Flavonoid, and Terpenoids were observed in PDT. The TLC finger-print chromatograms of PHT after development with n-Hexane:Ethyl acetate (3:2) showed four distinct components under ultraviolet light at 365 nm, and three spots when sprayed with 20% methanolic sulphuric under visible light, while, PDT developed in n-Hexane:ethyl acetate:methanol (2.5:2:0.5) revealed three fluorescent components at 365 nm and four components after sulphuric acid treatment.Conclusion: From this present study, identity cards have been designed for PHT and PDT through bioactive composition and TLC profiles which can be used in accessing the quality and consistency of the herbal drugs.  
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