Performances of an Electrochemical Detector using Prussian blue modified Indium Tin Oxide Electrode

An amperometric biosensor based on Prussian blue (PB) modified indium tin oxide (ITO) electrode was developed. The roughness of PB film which affects the electrocatalytic properties of PB film was controlled by varying deposition voltage and time. Atomic force microscopy (AFM) was used to measure the roughness of PB films deposited under various conditions. We used capillary electrophoresis (CE) method to make the analytes flow through the micro channel made of polydimethylsilolxane (PDMS). Compared with sensor using bare ITO electrode, the sensor using PB modified electrode has shown better sensitivity. In addition, we could improve the detection limit of the sensor by depositing smoother PB film