Talia Backman, Sergio M Latorre, Efthymia Symeonidi, Artur Muszyński, Ella Bleak, Lauren Eads, Paulina I Martinez-Koury, Sarita Som, Aubrey Hawks, Andrew D Gloss, David M Belnap, Allison M Manuel, Adam M Deutschbauer, Joy Bergelson, Parastoo Azadi, Hernán A Burbano, Talia L Karasov
{"title":"一种武器化噬菌体抑制了历史上和现代病原菌元种群中的竞争者。","authors":"Talia Backman, Sergio M Latorre, Efthymia Symeonidi, Artur Muszyński, Ella Bleak, Lauren Eads, Paulina I Martinez-Koury, Sarita Som, Aubrey Hawks, Andrew D Gloss, David M Belnap, Allison M Manuel, Adam M Deutschbauer, Joy Bergelson, Parastoo Azadi, Hernán A Burbano, Talia L Karasov","doi":"10.1101/2023.04.17.536465","DOIUrl":null,"url":null,"abstract":"<p><p>Bacteriophages, the viruses of bacteria, are proposed to drive bacterial population dynamics, yet direct evidence of their impact on natural populations is limited. Here we identified viral sequences in a metapopulation of wild plant-associated <i>Pseudomonas</i> spp. genomes. We discovered that the most abundant viral cluster does not encode an intact phage but instead encodes a tailocin - a phage-derived element that bacteria use to kill competitors for interbacterial warfare. Each pathogenic <i>Pseudomonas</i> sp. strain carries one of a few distinct tailocin variants, which target variable polysaccharides in the outer membrane of co-occurring pathogenic strains. Analysis of historic herbarium samples from the last 170 years revealed that the same tailocin and receptor variants have persisted in the <i>Pseudomonas</i> populations for at least two centuries, suggesting the continued use of a defined set of tailocin haplotypes and receptors. These results indicate that tailocin genetic diversity can be mined to develop targeted \"tailocin cocktails\" for microbial control.</p><p><strong>One-sentence summary: </strong>Bacterial pathogens in a host-associated metapopulation use a repurposed prophage to kill their competitors.</p>","PeriodicalId":22015,"journal":{"name":"Soil Science","volume":"167 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10862724/pdf/","citationCount":"0","resultStr":"{\"title\":\"A weaponized phage suppresses competitors in historical and modern metapopulations of pathogenic bacteria.\",\"authors\":\"Talia Backman, Sergio M Latorre, Efthymia Symeonidi, Artur Muszyński, Ella Bleak, Lauren Eads, Paulina I Martinez-Koury, Sarita Som, Aubrey Hawks, Andrew D Gloss, David M Belnap, Allison M Manuel, Adam M Deutschbauer, Joy Bergelson, Parastoo Azadi, Hernán A Burbano, Talia L Karasov\",\"doi\":\"10.1101/2023.04.17.536465\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Bacteriophages, the viruses of bacteria, are proposed to drive bacterial population dynamics, yet direct evidence of their impact on natural populations is limited. Here we identified viral sequences in a metapopulation of wild plant-associated <i>Pseudomonas</i> spp. genomes. We discovered that the most abundant viral cluster does not encode an intact phage but instead encodes a tailocin - a phage-derived element that bacteria use to kill competitors for interbacterial warfare. Each pathogenic <i>Pseudomonas</i> sp. strain carries one of a few distinct tailocin variants, which target variable polysaccharides in the outer membrane of co-occurring pathogenic strains. Analysis of historic herbarium samples from the last 170 years revealed that the same tailocin and receptor variants have persisted in the <i>Pseudomonas</i> populations for at least two centuries, suggesting the continued use of a defined set of tailocin haplotypes and receptors. These results indicate that tailocin genetic diversity can be mined to develop targeted \\\"tailocin cocktails\\\" for microbial control.</p><p><strong>One-sentence summary: </strong>Bacterial pathogens in a host-associated metapopulation use a repurposed prophage to kill their competitors.</p>\",\"PeriodicalId\":22015,\"journal\":{\"name\":\"Soil Science\",\"volume\":\"167 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-02-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10862724/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Soil Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1101/2023.04.17.536465\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Soil Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1101/2023.04.17.536465","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
A weaponized phage suppresses competitors in historical and modern metapopulations of pathogenic bacteria.
Bacteriophages, the viruses of bacteria, are proposed to drive bacterial population dynamics, yet direct evidence of their impact on natural populations is limited. Here we identified viral sequences in a metapopulation of wild plant-associated Pseudomonas spp. genomes. We discovered that the most abundant viral cluster does not encode an intact phage but instead encodes a tailocin - a phage-derived element that bacteria use to kill competitors for interbacterial warfare. Each pathogenic Pseudomonas sp. strain carries one of a few distinct tailocin variants, which target variable polysaccharides in the outer membrane of co-occurring pathogenic strains. Analysis of historic herbarium samples from the last 170 years revealed that the same tailocin and receptor variants have persisted in the Pseudomonas populations for at least two centuries, suggesting the continued use of a defined set of tailocin haplotypes and receptors. These results indicate that tailocin genetic diversity can be mined to develop targeted "tailocin cocktails" for microbial control.
One-sentence summary: Bacterial pathogens in a host-associated metapopulation use a repurposed prophage to kill their competitors.
期刊介绍:
Cessation.Soil Science satisfies the professional needs of all scientists and laboratory personnel involved in soil and plant research by publishing primary research reports and critical reviews of basic and applied soil science, especially as it relates to soil and plant studies and general environmental soil science.
Each month, Soil Science presents authoritative research articles from an impressive array of discipline: soil chemistry and biochemistry, physics, fertility and nutrition, soil genesis and morphology, soil microbiology and mineralogy. Of immediate relevance to soil scientists-both industrial and academic-this unique publication also has long-range value for agronomists and environmental scientists.