一种武器化噬菌体抑制了历史上和现代病原菌元种群中的竞争者。

4区 农林科学 Q2 Agricultural and Biological Sciences Soil Science Pub Date : 2024-02-02 DOI:10.1101/2023.04.17.536465
Talia Backman, Sergio M Latorre, Efthymia Symeonidi, Artur Muszyński, Ella Bleak, Lauren Eads, Paulina I Martinez-Koury, Sarita Som, Aubrey Hawks, Andrew D Gloss, David M Belnap, Allison M Manuel, Adam M Deutschbauer, Joy Bergelson, Parastoo Azadi, Hernán A Burbano, Talia L Karasov
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引用次数: 0

摘要

噬菌体是细菌的病毒,被认为是细菌种群动态的驱动力,但它们对自然种群影响的直接证据却很有限。在这里,我们确定了野生植物相关假单胞菌属基因组元群中的病毒序列。我们发现,最丰富的病毒簇并不编码完整的噬菌体,而是编码一种噬菌体衍生元素--尾蛋白,细菌利用这种元素杀死竞争者,进行细菌间战争。每种致病假单胞菌株都携带几种不同的尾孢菌素变体之一,这些变体的目标是共生致病菌株外膜上的可变多糖。对过去 170 年历史标本馆样本的分析表明,相同的尾丝菌素和受体变体在假单胞菌种群中至少存在了两个世纪,这表明一组确定的尾丝菌素单倍型和受体仍在继续使用。这些结果表明,可以利用尾孢素遗传多样性来开发有针对性的 "尾孢素鸡尾酒",以控制微生物。
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A weaponized phage suppresses competitors in historical and modern metapopulations of pathogenic bacteria.

Bacteriophages, the viruses of bacteria, are proposed to drive bacterial population dynamics, yet direct evidence of their impact on natural populations is limited. Here we identified viral sequences in a metapopulation of wild plant-associated Pseudomonas spp. genomes. We discovered that the most abundant viral cluster does not encode an intact phage but instead encodes a tailocin - a phage-derived element that bacteria use to kill competitors for interbacterial warfare. Each pathogenic Pseudomonas sp. strain carries one of a few distinct tailocin variants, which target variable polysaccharides in the outer membrane of co-occurring pathogenic strains. Analysis of historic herbarium samples from the last 170 years revealed that the same tailocin and receptor variants have persisted in the Pseudomonas populations for at least two centuries, suggesting the continued use of a defined set of tailocin haplotypes and receptors. These results indicate that tailocin genetic diversity can be mined to develop targeted "tailocin cocktails" for microbial control.

One-sentence summary: Bacterial pathogens in a host-associated metapopulation use a repurposed prophage to kill their competitors.

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来源期刊
Soil Science
Soil Science 农林科学-土壤科学
CiteScore
2.70
自引率
0.00%
发文量
0
审稿时长
4.4 months
期刊介绍: Cessation.Soil Science satisfies the professional needs of all scientists and laboratory personnel involved in soil and plant research by publishing primary research reports and critical reviews of basic and applied soil science, especially as it relates to soil and plant studies and general environmental soil science. Each month, Soil Science presents authoritative research articles from an impressive array of discipline: soil chemistry and biochemistry, physics, fertility and nutrition, soil genesis and morphology, soil microbiology and mineralogy. Of immediate relevance to soil scientists-both industrial and academic-this unique publication also has long-range value for agronomists and environmental scientists.
期刊最新文献
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