Yifei Wang, W. Yuan, Qinming Zhang, Yixuan Wang, M. Kimber, Liang Dong, Meng Lu
{"title":"基于无标记成像生物传感器的外泌体微阵列","authors":"Yifei Wang, W. Yuan, Qinming Zhang, Yixuan Wang, M. Kimber, Liang Dong, Meng Lu","doi":"10.1109/SENSORS43011.2019.8956490","DOIUrl":null,"url":null,"abstract":"Exosome vesicles (EVs) released by macrophages are potential biomarkers for the analysis of immune responses. This study reports a high-throughput EV detection assay developed using a label-free EV microarray. The EV microarray consists of a panel of seven antibodies that are specific to multiple membrane receptors of the target EVs. The EV microarray was fabricated on a photonic crystal (PC) biosensor surface. The hyperspectral imaging approached was implemented to quantify the antibody and EV absorptions on the PC-based microarray. The label-free EV microarray enables low-cost, rapid, and high-throughput characterization of macrophage EVs with a significantly reduced sample volume of 1 μL.","PeriodicalId":6710,"journal":{"name":"2019 IEEE SENSORS","volume":"6 1","pages":"1-4"},"PeriodicalIF":0.0000,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Exosome microarray based on label-free imaging biosensor\",\"authors\":\"Yifei Wang, W. Yuan, Qinming Zhang, Yixuan Wang, M. Kimber, Liang Dong, Meng Lu\",\"doi\":\"10.1109/SENSORS43011.2019.8956490\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Exosome vesicles (EVs) released by macrophages are potential biomarkers for the analysis of immune responses. This study reports a high-throughput EV detection assay developed using a label-free EV microarray. The EV microarray consists of a panel of seven antibodies that are specific to multiple membrane receptors of the target EVs. The EV microarray was fabricated on a photonic crystal (PC) biosensor surface. The hyperspectral imaging approached was implemented to quantify the antibody and EV absorptions on the PC-based microarray. The label-free EV microarray enables low-cost, rapid, and high-throughput characterization of macrophage EVs with a significantly reduced sample volume of 1 μL.\",\"PeriodicalId\":6710,\"journal\":{\"name\":\"2019 IEEE SENSORS\",\"volume\":\"6 1\",\"pages\":\"1-4\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2019 IEEE SENSORS\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/SENSORS43011.2019.8956490\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2019 IEEE SENSORS","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/SENSORS43011.2019.8956490","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Exosome microarray based on label-free imaging biosensor
Exosome vesicles (EVs) released by macrophages are potential biomarkers for the analysis of immune responses. This study reports a high-throughput EV detection assay developed using a label-free EV microarray. The EV microarray consists of a panel of seven antibodies that are specific to multiple membrane receptors of the target EVs. The EV microarray was fabricated on a photonic crystal (PC) biosensor surface. The hyperspectral imaging approached was implemented to quantify the antibody and EV absorptions on the PC-based microarray. The label-free EV microarray enables low-cost, rapid, and high-throughput characterization of macrophage EVs with a significantly reduced sample volume of 1 μL.
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