含钾氧化钛纳米颗粒调节人树突状细胞对麻风分枝杆菌的免疫反应

IF 2.1 Q3 MICROBIOLOGY Microbiology Research Pub Date : 2023-06-13 DOI:10.3390/microbiolres14020055
Sam A. Warren, So Yoon Lee, J. Barragan, P. Kositangool, Hatsuko Yoshikubo, J. Cervantes
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引用次数: 0

摘要

麻风病的两种极端临床形式,称为结核性和麻风性,具有极化的细胞免疫反应,具有复杂的免疫学差异。据报道,结核性麻风病中dc占主导地位,而麻风病的模式与当地dc人群的弱激活有关。二氧化钛纳米颗粒先前已被证明可以诱导这些细胞成熟,导致类似于疫苗佐剂使用的炎症反应。我们旨在评估钾结合的氧化钛纳米结构,即KTiOxs,在人单核细胞来源的dc对活麻风分枝杆菌的反应中的作用。人单核细胞系双THP-1携带NF-κB转录因子激活和干扰素调节因子(IRF)两个可诱导的报告质粒系统,分别用对照钛或1 mol/L KOH处理过的Ti或10 mol/L KOH处理24 h,然后感染麻风分枝杆菌。KTiOx纳米颗粒在无炎症的情况下增加DC吞噬活性。dc的KTiOx暴露导致IRF激活增加,并调节对活麻风分枝杆菌的炎症反应。它还导致先天免疫反应关键成分的差异分泌和细胞介导的针对细胞内病原体的免疫的发展。这项研究证明了KTiOxs纳米结构的作用,以及纳米颗粒技术在体外激活人类dc对抗一种具有令人困惑的免疫谱的传染病方面的有用性。我们的发现可能会促进未来的治疗策略,如DC免疫治疗弥散性和进行性麻风病变。
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Potassium-Incorporated Titanium Oxide Nanoparticles Modulate Human Dendritic Cell Immune Response to Mycobacterium leprae
The two polar clinical forms of leprosy, termed tuberculoid and lepromatous, have polarized cellular immune responses with complex immunological distinctions. The predominance of DCs in tuberculoid leprosy has been reported, while the lepromatous pattern of illness is associated with weak activation of local populations of DCs. TiO2 nanoparticles have previously been shown to induce maturation of these cells, leading to an inflammatory response similar to adjuvant usage in vaccine administration. We aimed to evaluate the effect of potassium-incorporated Ti oxide nanostructures, namely KTiOxs, in the response of human monocyte-derived DCs to live M. leprae. Human monocytic cell line dual THP-1, which harbors two inducible reporter plasmid systems for transcription factor activation of NF-κB and interferon regulating factor (IRF), was treated with titanium control or with 1 mol/L KOH-treated Ti or 10 mol/L KOH for 24 h. Subsequently, cells were infected with M. leprae. KTiOx nanoparticles increase DC phagocytic activity without inflammation. KTiOx exposure of DCs led to an increase in IRF activation with modulation of the inflammatory response to live M. leprae. It also led to differential secretion of the critical components of innate immune response and the development of cell-mediated immunity against intracellular pathogens. This study demonstrates the effect of nanostructures of KTiOxs and the usefulness of nanoparticle technology in the in vitro activation of human DCs against an infectious disease with a puzzling immune spectrum. Our findings may prompt future therapeutic strategies, such as DC immunotherapy for disseminated and progressive lepromatous lesions.
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来源期刊
Microbiology Research
Microbiology Research MICROBIOLOGY-
CiteScore
1.90
自引率
6.70%
发文量
62
审稿时长
10 weeks
期刊介绍: Microbiology Research is an international, online-only, open access peer-reviewed journal which publishes original research, review articles, editorials, perspectives, case reports and brief reports to benefit researchers, microbiologists, physicians, veterinarians. Microbiology Research publishes ‘Clinic’ and ‘Research’ papers divided into two different skill and proficiency levels: ‘Junior’ and ‘Professional’. The aim of this four quadrant grid is to encourage younger researchers, physicians and veterinarians to submit their results even if their studies encompass just a limited set of observations or rely on basic statistical approach, yet upholding the customary sound approach of every scientific article.
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