白鲈鱼诱导二倍体雌核发生

B. Gomelsky, N. Cherfas, A. Gissis, G. Hulata
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引用次数: 15

摘要

摘要确定了白鲈鱼二倍体雌核分裂诱导的最佳条件。通过两个系列的实验,确定了紫外线照射灭活精子基因组的剂量和恢复二倍体的最佳温度冲击。用盐水稀释(1:30)的萨氏条纹鲈鱼精子对白鲈鱼卵进行受精,并用50 ~ 1200 J/m2剂量的紫外线照射。随着紫外线剂量的增加,观察到典型的“Hertwig效应”。在400 J/m2及以上剂量下获得的幼虫发育异常(单倍体综合征),染色体数目为单倍体(n = 24)。热冲击引起第二极体的保留。在授精后2或3分钟,分别在36°C、38°C或40°C条件下对辐照(800 J/m2)条纹鲈鱼精子受精的白鲈鱼卵施加持续2分钟的热冲击。在人工授精后3 min施加36℃热休克,效果最好。
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Induced Diploid Gynogenesis in White Bass
Abstract Optimal conditions were determined for induction of meiotic diploid gynogenesis in white bass Morone chrysops. In two series of experiments, ultraviolet light (UV) irradiation doses to inactivate sperm genome and optimal temperature shock to restore diploidy were established. Eggs of white bass were inseminated with sperm from striped bass M. saxatilis diluted (1:30) in saline and irradiated with UV at doses ranging from 50 to 1,200 J/m2. A typical “Hertwig effect” was observed with increasing UV dose. Larvae obtained at doses of 400 J/m2 and greater were abnormally developed (haploid syndrome) and had a haploid chromosome number (n = 24). Heat shocks to cause retention of the second polar body were applied. Heat shocks of 2-min duration at 36°C, 38°C, or 40°C were applied at 2 or 3 min after insemination to white bass eggs inseminated with irradiated (800 J/m2) striped bass sperm. The best results were obtained after application of 36°C heat shock at 3 min after insemination, which induced a sig...
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