pef辅助提取红树莓花青素的研究

Luo Wei, Zhang Ruobing, W. Liming, Chen Jie, Guan Zhicheng, L. Xiaojun, Mo Mengbin
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引用次数: 6

摘要

脉冲电场是一种新型的非热加工技术。由于其在植物细胞膜上的不可逆分解作用,可用于植物功能成分的提取。PEF处理对覆盆子细胞膜产生不可逆的崩解作用,覆盆子花青素提取率与PEF处理脉冲呈线性相关。在提取过程中,覆盆子中的主要花青素花青素-3-葡萄糖苷被PEF显著降解。随着电场的增大和处理时间的延长,花青素-3-葡萄糖苷的降解效果逐渐降低。在经PEF处理的花青素-3-葡萄糖苷的光谱曲线中,333 nm处的峰强度随着电场和处理时间的增加而增加,519 nm处的峰强度和430 nm处的肩峰强度下降,说明花青素-3-葡萄糖苷被降解为查尔酮。
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Effects of PEF-assisted extraction of anthocyanin in red raspberry
Pulsed electric field is a novel non-thermal process technology. It could be applied in the extraction of functional components from plant for its irreversible disintegration on plant cell membrane. PEF treatment caused an irreversible disintegration on the cellular membrane of raspberry cell and the extraction yield of anthocyanins from raspberry was linearly correlated to the treatment pulses of PEF. During the extraction, Cyanidin-3-glucoside, the major anthocyanins in raspberry was found to be significantly degraded by PEF. The degradation effects of Cyanidin-3-glucoside decreased with the increase of the electric field or treatment time. In the spectra curves of Cyanidin-3-glucoside treated by PEF, the intensity of the peak at 333 nm increased as electric field and treatment time increased, while the intensity of the peak at 519 nm and the shoulder at 430 nm decreased, indicating Cyanidin-3-glucoside was degraded into chalcone.
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