印度尼西亚几种商用阿拉比卡咖啡豆的特性

Y. Desmiaty, Liliek Nurhidayati, N. M. D. Sandhiutami, Reczky Muhammad Ramdhan Hasan, Kharinta Adella Meynderth, Dianita Ayu Noviastuti
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引用次数: 0

摘要

阿拉比卡咖啡是印尼种植业的主要商品之一。地理差异和咖啡生长环境会影响咖啡豆的特性、植物化学成分和抗氧化活性。本研究的目的是测定10种印度尼西亚市售阿拉比卡咖啡豆的植物化学特性和抗氧化活性。采用70%乙醇动态浸渍1小时提取10颗咖啡豆,然后蒸发。对各提取物进行形态检测、植物化学筛选、HPLC法测定咖啡因含量、黄烷-3-醇含量、总黄酮含量(TFC)、总多酚含量(TPC)、DPPH法和FRAP法测定抗氧化性。所有的豆类样品都含有类黄酮、生物碱、三萜、香豆素和单宁。化学含量测定:咖啡因含量为1.5 ~ 2.9%;黄烷-3-醇含量4.85 ~ 12.38cat/g提取物;TFC 9.71-23.67QE /g提取物;TPC 21.8 ~ 76.26 gae /g提取物。使用DPPH进行的抗氧化能力测试显示,IC₅₀值从19.49-81.41g/mL变化具有抑制作用,使用FRAP方法给出IC₅₀值从18.01-61.2g/mL。该研究表明,咖啡样品具有相同的植物化学特性,并且具有重要的抗氧化活性,这证明了其开发成抗氧化营养保健品的潜力。
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The Characteristics of Some Commercial Arabica Coffee Beans in Indonesia
Arabica coffee is one of the mainstay commodities in the Indonesian plantation sector. Geographical differences and the environment where coffee grow can affect the characteristic, phytochemical content and antioxidant activity of the beans. The aim of this study was to determine the phytochemical characteristics and antioxidant activity of 10 items of commercial Arabica coffee beans in Indonesia. The extraction of 10 coffee beans was carried out by kinetic maceration in 1 hour with 70% ethanol, then evaporated. Each extract was examined for morphology, phytochemical screening, assay of caffeine content using HPLC method, levels of flavan-3-ol, total flavonoid content (TFC), total polyphenolic content (TPC), antioxidant determination using DPPH and FRAP methods. All bean samples showed the presence of flavonoids, alkaloids, triterpenoids, coumarins, and tannins. The chemical content determination showed caff eine content of 1.5-2.9%; flavan-3-ol content 4.85-12.38cat/g extract; TFC 9.71–23.67QE/g extract; and TPC 21.8–76.26GAE/g extract. Tests of antioxidant capacity using the DPPH reveal an inhibiting effect with varying IC₅₀ values from 19.49-81.41g/mL and using the FRAP method gave IC₅₀ values from 18.01-61.2g/mL. This study indicates that coffee samples have the same phytochemical characteristics and also have an important antioxidant activity, which justifies its potential to be developed into antioxidant nutraceuticals.
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审稿时长
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