铜绿假单胞菌KGS促进十二烷基硫酸钠生物降解的最佳条件

R. Shahbazi, R. Kasra-Kermanshahi, S. Gharavi, Z. M. Nejad, Faezeh Borzooee
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引用次数: 1

摘要

利用铜绿假单胞菌KGS菌株对阴离子表面活性剂十二烷基硫酸钠(SDS)进行降解。JQ328193,从洗车废水中分离得到。以十二烷基硫酸钠铜绿假单胞菌(P. aeruginosa kgs)为研究对象,通过亚甲基蓝活性物质(MBAS)法测定SDS的降解率,考察氮碳源、pH、温度、接种量和不同SDS浓度等不同理化因素对SDS生物降解的影响。该菌株对SDS的最佳降解条件为:1.5mM SDS,接种率7%,pH 7.5,温度37℃,硝酸铵(氮源),基础盐培养基中添加葡萄糖作为共底物。在优化的生物降解条件下,培养24小时后,该细菌可降解约98%的SDS。本研究结果表明,铜绿假单胞菌是SDS生物降解的合适候选者。
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Optimal conditions for enhancing sodium dodecyl sulfate biodegradation by Pseudomonas aeruginosa KGS
The anionic surfactant sodium dodecyl sulfate (SDS) was degraded by novel strain ofPseudomonas aeruginosa KGS under accession No. JQ328193, which was isolated from carwash wastewater. The purpose of this research was to study different optimization conditionsrequired for enhancing the biodegradation of sodium dodecyl sulfate P. aeruginosa KGS.Influence of different Physicochemical factors such as nitrogen and carbon sources, pH,temperature, inoculation percent and different concentrations of SDS on the biodegradation ofSDS were investigated by measuring the degradation rate of SDS using methylene blue activesubstance (MBAS) method. The optimum conditions determined for the this selected bacteriumstrain for degradation of SDS were 1.5mM SDS, inoculation percent 7%, pH 7.5, temperature37°C, ammonium nitrate (nitrogen source) when basal salt medium was supplemented withglucose as a co substrate. This bacterium is able to degrade about 98% of the SDS after 24h ofincubation under optimized conditions of biodegradation. The results presented in this researchindicate that Pseudomonas aeruginosa is a suitable candidate for SDS biodegradation.
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