用于非漂白和非闪烁光学生物成像的单层二硫化钼纳米探针的双光子发光和二次谐波产生

Qiuqiang Zhan, Xin Zhang, Baoju Wang, Nana Li, Sailing He
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引用次数: 6

摘要

层状二硫化钼(MoS2)在可见光激发下可以有效地发出光致发光(PL)。然而,用于生物成像目的的二硫化钼的单光子发光受到强自身荧光和离子诱导的发光猝灭的影响。本文报道了单层壳聚糖修饰的二硫化钼纳米片在近红外飞秒激光激发下的非漂白和非闪烁光学纳米探针及其在双光子发光(TPL)和二次谐波产生(SHG)生物成像中的应用。TPL能抵抗离子诱导的细胞膜猝灭。所提出的单层二硫化钼的TPL和SHG在实时、深度和多光子生物成像方面显示出巨大的潜力。
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TWO-PHOTON LUMINESCENCE AND SECOND HARMONIC GENERATION OF SINGLE LAYER MOLYBDENUM DISULPHIDE NANOPROBE FOR NONBLEACHING AND NONBLINKING OPTICAL BIOIMAGING
Layered molybdenum disulphide (MoS2) can efficiently emit photoluminescence (PL) excited by visible light. However, one-photon PL of MoS2 for bioimaging purposes suffers from strong autofluorescence and ion-induced PL quenching. Herein, we report single layer chitosan decorated MoS2 nanosheets as nonbleaching and nonblinking optical nanoprobes under near infrared femtosecond laser excitation and their applications for two photon luminescence (TPL) and second harmonic generation (SHG) bioimaging. The TPL can resist the ion-induced quenching by the cellular membrane. The proposed TPL and SHG of single layer MoS2 show great potential for real-time, deep and multiphoton bioimaging.
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