P53在振荡剪切应力诱导EPCs向平滑肌细胞转分化中的作用

Q4 Biochemistry, Genetics and Molecular Biology Molecular & Cellular Biomechanics Pub Date : 2019-02-21 DOI:10.32604/mcb.2019.05758

Yu Gao, Meiyue Wang, Yanting He, Lanlan Li, Xiaodong Cui, Min Cheng, Xiaoyun Zhang

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引用次数: 1

摘要

本研究探讨了P53在振荡剪切应力诱导内皮祖细胞(EPCs)向平滑肌细胞转分化中的作用。将内皮祖细胞(EPCs)置于载玻片上，施加4达因/平方厘米的振荡剪切应力(OSS)。结果显示，OSS后P53的表达呈时间依赖性降低。OSS还能减弱内皮细胞标志物vWF和CD31的表达，但能增强EPCs中平滑肌细胞标志物α-SMA和SM22的表达。用P53激动剂预处理EPCs后，采用基质凝胶法检测体外血管生成的变化，Western blot法检测α - sma和SM22的表达。结果表明，单纯振荡剪应力能降低EPCs的血管生成能力，而P53激动剂能提高EPCs的血管生成能力，下调α-SMA和SM22的表达。根据以上结果，我们推测P53可能在OSS诱导EPCs向平滑肌细胞转分化过程中发挥作用。

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The Role of P53 in Transdifferentiation of EPCs into Smooth Muscle Cells Induced by Oscillatory Shear Stress

This study examines the effects of P53 in transdifferentiation of endothelial progenitor cells (EPCs) into smooth muscle cells induced by oscillatory shear stress. Endothelial progenitor cells (EPCs) were planted on slide and treated with 4 dyne/cm2 oscillatory shear stress (OSS). Results showed that the expression P53 was decreased time dependent after OSS. The OSS also attenuated the endothelial cells marker vWF and CD31 expression but enhanced the marker of smooth muscle cell α-SMA and SM22 expression in EPCs. After EPCs were pretreated with P53 agonist, the changes of angiogenesis in vitro were detected by matrix gel, and the expressions of alpha-SMA and SM22 were detected by Western blot. The results showed that simple oscillatory shear stress could decrease but P53 agonist could improve the ability of angiogenesis on EPCs, and down-regulate the expression of α-SMA and SM22. From the above results, we speculate that P53 may play a role in the transdifferentiation of EPCs into smooth muscle cells induced by OSS.

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来源期刊

Molecular & Cellular Biomechanics CELL BIOLOGYENGINEERING, BIOMEDICAL&-ENGINEERING, BIOMEDICAL

CiteScore

1.70

自引率

0.00%

发文量

期刊介绍： The field of biomechanics concerns with motion, deformation, and forces in biological systems. With the explosive progress in molecular biology, genomic engineering, bioimaging, and nanotechnology, there will be an ever-increasing generation of knowledge and information concerning the mechanobiology of genes, proteins, cells, tissues, and organs. Such information will bring new diagnostic tools, new therapeutic approaches, and new knowledge on ourselves and our interactions with our environment. It becomes apparent that biomechanics focusing on molecules, cells as well as tissues and organs is an important aspect of modern biomedical sciences. The aims of this journal are to facilitate the studies of the mechanics of biomolecules (including proteins, genes, cytoskeletons, etc.), cells (and their interactions with extracellular matrix), tissues and organs, the development of relevant advanced mathematical methods, and the discovery of biological secrets. As science concerns only with relative truth, we seek ideas that are state-of-the-art, which may be controversial, but stimulate and promote new ideas, new techniques, and new applications.