蛙蛙声囊的形态学和免疫组织化学研究

M. Inceli, S. Sancar-Bas, Engin Kaptan
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摘要

本研究的目的是研究雄性ridibunda的声囊结构,并在光镜下免疫组织化学检测其组成部分中泌乳素受体的存在。ridibunda的声囊位于双侧口腔两侧,包含两层不同的鞘,即内鞘和外鞘。外鞘和内鞘分别由多层鳞状角质化上皮和单层柱状上皮组成,很少有纤毛细胞。内鞘上皮下的结缔组织以骨骼肌纤维为主。此外,在外鞘表皮最上层细胞层观察到催乳素受体的免疫反应性。本文是首次研究双侧声囊的形态学特征及其激素相互作用的研究之一。我们认为,这项研究对两栖动物生殖生物学领域也有贡献。
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Vocal Sac of Rana ridibunda: A Morphological and Immunohistochemical Study
The goal of this study is to investigate the structure of the vocal sac in males of Rana ridibunda and to detect the presence of prolactin receptor immunohistochemically under light microscopy in its components The vocal sac of Rona ridibunda were bilaterally localized on each side of the mouth and contained two different layers, namely an internal and an external sheath. External and internal sheaths were formed with multilayered squamous keratinized epithelium and single layered columnar epithelium with rarely ciliated cells, respectively. The connective tissue under the epithelium in the internal sheath possessed predominantly skeletal muscle fibers. Furthermore, the prolactin receptor immunoreactivity was observed in the uppermost cell layer of the epidermis in the external sheath. This paper is one of the first studies for the purpose of displaying the morphological aspects of the bilaterally located vocal sac and its hormonal interaction. In our opinion, this study also contributes to the field of amphibian reproductive biology.
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