James P. Meador , Suzanne C. Ball , C. Andrew James , Jenifer K. McIntyre
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引用次数: 0
摘要
对暴露于大型城市污水处理厂废水(WWE)中 10 天的奇努克鲑幼鱼进行了目标代谢组学分析。暴露包括五种 WWE 稀释液(20%、5.3%、1.4%、0.4% 和 0.1%)和一种对照,每种处理有 7 个重复。提取鱼肝,通过液相色谱-质谱法(LC-MS)分析 361 种内源性代谢物,其中检测到 185 种。对照组与处理组的比较确定了几种代谢物,这些代谢物与所有处理组观察到的生化途径改变有关,其中包括几种对能量生成和利用很重要的代谢物。这些改变的途径对鱼类健康至关重要,可能是对生长、繁殖、行为和免疫功能产生潜在不利影响的早期指标。海洋型大鳞大麻哈鱼幼鱼在近岸河口停留数天至数周,可能会遇到高浓度的 WWE 污染物。它们会接触到来自 WWE 的各种强效药品、个人护理产品和工业化合物,这些物质可能会影响生理平衡并破坏其正常的生命周期。
Exposure of juvenile Chinook salmon to effluent from a large urban wastewater treatment plant. Part 2. Metabolomic profiling
Targeted metabolomic analysis was conducted on juvenile Chinook salmon exposed for 10 days to wastewater effluent (WWE) from a large urban treatment plant. Exposures included five dilutions of WWE (20%, 5.3%, 1.4%, 0.4%, and 0.1%) and a control with 7 replicates per treatment. Liver was extracted from fish and analyzed via liquid chromatography – mass spectrometry (LC-MS) for 361 endogenous metabolites, of which 185 were detected. Control-versus-treatment comparisons identified several metabolites that were associated with altered biochemical pathways observed for all treatments, including several that are important for energy generation and utilization. These altered pathways are crucial for fish health and may be an early indicator of potential adverse effects on growth, reproduction, behavior, and immune function. Juvenile ocean-type Chinook salmon spend several days to weeks in the nearshore estuary where they may encounter high concentrations of WWE contaminants. They are exposed to a wide range of potent pharmaceuticals, personal care products, and industrial compounds from WWE that have the potential to affect physiological homeostasis and disrupt their normal life cycle.