胶体纳米银的体外抗菌活性

T. Popova, I. Ignatov
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引用次数: 3

摘要

研究了胶体纳米银(AgNPs)在20和30 ppm浓度下对大肠杆菌、肠炎沙门氏菌、金黄色葡萄球菌、产气荚膜芽胞杆菌、白色念珠菌和两种临床分离菌株(铜绿假单胞菌和化脓性链球菌)的抑菌效果。采用经典的琼脂扩散法,测定AgNPs的最低抑菌浓度(MIC)和抑菌时间。在琼脂扩散法进行的研究中,30 ppm的AgNPs和20 ppm的AgNPs对所有研究的微生物都有很好的抑制效果。革兰氏阴性菌对两种制剂的敏感性均高于革兰氏阳性菌(P < 0.05)。金黄色葡萄球菌和白色念珠菌的敏感性最低,铜绿假单胞菌和化脓性葡萄球菌的敏感性最高。据报道,铜绿假单胞菌和产气荚膜假单胞菌的AgNPs最低mic为20 ppm。MIC50为1 μg/mL,其余为- 2 μg/mL。同样,产气荚膜球菌在MIC50为0.5 μg/mL时对AgNPs 30 ppm的敏感性最高,金黄色葡萄球菌在MIC50为2 μg/mL时敏感性最低。革兰氏阳性菌的MIC值高于革兰氏阴性菌。AgNPs 20 ppm和AgNPs 30 ppm在最终浓度为103个细胞/mL和104个细胞/mL时,在5分钟内灭活所有菌株;只有白色念珠菌存活时间更长。当AgNPs浓度为20 ppm和30 ppm时,被测微生物在浓度为106个细胞/mL的混悬液中存活的时间明显更长。这些结果有望成功地将AgNPs用于消毒,以及用于涉及这些细菌物种的感染的局部治疗。
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In vitro antimicrobial activity of colloidal nano silver
The antimicrobial effect of colloidal nanosilver (AgNPs) at concentrations of 20 and 30 ppm against reference Esherichia coli, Salmonella enterica, Staphylococcus aureus, Clostridium perfringens, Candida albicans strains and two clinical isolates (Pseudominas aeruginosa and Streptococcus pyogenes) was tested. The classical agar diffusion method, determination of the minimum inhibitory concentrations (MIC) and the time of antimicrobial action of AgNPs were used. In the studies performed by the agar diffusion method, a very good inhibitory effect of AgNPs 30 ppm and to a much lesser extent of AgNPs 20 ppm, was reported against all studied microorganisms. The studied Gram-negative bacteria showed higher sensitivity to both preparations compared to the Gram-positive microorganisms (P>0.05). The lowest sensitivity was reported for S. aureus and C. albicans, and the highest - for P. aeruginosa and S. pyogenes. The lowest MICs of AgNPs 20 ppm were reported for P. aeruginosa and C. perfringens. For them, the MIC50 was 1 μg/mL, and for the rest - 2 μg/mL. Again, C. perfringens showed the highest sensitivity to AgNPs 30 ppm with MIC50 0.5 μg/mL, and the lowest one was that of S. aureus with MIC50 2 μg/mL. For the Gram-positive bacteria MIC values were higher than for Gram-negative ones. AgNPs 20 ppm and AgNPs 30 ppm inactivated all bacterial strains tested at final concentrations of 103 cells/mL and 104 cells/mL within 5 min; only C. albicans persisted longer. The tested microorganisms remained viable for significantly longer time in the presence of AgNPs 20 ppm and AgNPs 30 ppm when in suspensions with a density of 106 cells/mL. These results are promising for the successful use of AgNPs for disinfection, as well as for topical therapy of infections involving these bacterial species.
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来源期刊
BULGARIAN JOURNAL OF VETERINARY MEDICINE
BULGARIAN JOURNAL OF VETERINARY MEDICINE Veterinary-Veterinary (all)
CiteScore
1.00
自引率
0.00%
发文量
26
审稿时长
15 weeks
期刊介绍: BJVM is a no-fee open-access scientific quarterly journal which covers topics related to both fundamental and applied aspects of veterinary medicine and to closely connected subjects with it. The journal publishes original papers, short communications and reviews.
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