凤眼山冠犀鸟巢的霉菌多样性

Q3 Agricultural and Biological Sciences Biotropia Pub Date : 2022-04-24 DOI:10.11598/btb.2022.29.1.1615
M. Rahayuningsih, Yanuar Revandi, Harnina Bintari
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引用次数: 0

摘要

众所周知,环犀鸟(Rhyticeros zlndulates)在三个洞穴中筑巢,在那里它们设法生活和繁殖。预计这座大厦将包含维持微环境稳定性所需的各种模具。本研究旨在利用从没有鸟类活动的空结构中收集的样本,确定环犀鸟巢穴中的霉菌多样性。样品分别于2016年和2017年两次在IWsidi和Nglimut观测站获取。此外,样品包括覆盖土壤,木材和内部材料,这些都是无菌收集的,并放置在无菌自封塑料袋中。然后将这些样品用无菌蒸馏水稀释至10-3 mg/mL,随后接种于马铃薯葡萄糖琼脂(PDA)、麦芽提取物琼脂(MEA)和Czapek Dox琼脂(CDA)上。接种剂37℃孵育,第11天观察菌落情况。结果在Icalisidi和Nglimut观测站分别鉴定出7种和9种霉菌。在整个观察期的堆肥巢料中发现的种类最多的是Penin'llizlm sp.。
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MOLD DIVERSITY OF WREATHED HORNBILL (Rhyticeros undulatus) NEST IN MOUNT UNGARAN
Wreathed Hornbill (Rhyticeros zlndulates) is known to build nests in three cavities where they managed to live and breed. This edifice is predicted to contain various molds needed to maintain micro-environmental steadiness. This study was aimed to identify molds diversity in the Wreathed Hornbill's nest, using samples collected from empty structure with no bird activity. The samples were obtained from the IWsidi and Nglimut observation stations on two occasions, i.e., in 2016 and 2017. Furthermore, the samples comprised cover soil, wood and inner material, which were collected aseptically and placed in sterile ziplock plastic bags. These samples were then diluted in sterilized distilled water to attain 10-3 mg/mL, and subsequently inoculated on Potato Dextrose Agar (PDA), Malt Extract Agar (MEA) and Czapek Dox Agar (CDA). The inoculants were incubated at 37 OC, followed by the observation of mold colony after the 11 th day. The results identified seven and nine species of molds in the Icalisidi and Nglimut observation stations, respectively. The most abundant species was Penin'llizlm sp. which was found in composted nest materials for the whole observation periods.
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来源期刊
Biotropia
Biotropia Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
0.70
自引率
0.00%
发文量
23
审稿时长
30 weeks
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