姜黄的植物化学筛选及抑菌活性研究

P. Chauhan, Kruti Keni, Raxita D Patel
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引用次数: 12

摘要

许多植物的抗菌活性和药性已被筛选。本研究的目的是鼓励使用香料作为替代或补充药物,以减轻高昂的费用、副作用和逐渐增加的病原体耐药性的负担。以姜黄根茎为原料,分别制备了鲜姜黄和干姜黄的甲醇和氯仿提取物。通过对两种提取物的抑菌活性和抗真菌活性进行研究。采用琼脂孔扩散法对大肠杆菌、金黄色葡萄球菌、肺炎克雷伯菌、普通蛋白酶、铜绿假单胞菌、蜡样芽孢杆菌、巨型芽孢杆菌、枯草芽孢杆菌等细菌和黑曲霉等真菌进行抑菌试验。测定其抑菌范围,并与标准药物链霉素进行比较,了解其抑菌效果。与氯仿等溶剂相比,用甲醇提取根茎的效果更好。两种提取物的植物化学分析显示存在生物碱、类黄酮、萜类、单宁、皂苷和类固醇。
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Investigation of phytochemical screening and antimicrobial activity of Curcuma longa
Many plants have been screened for antimicrobial activity and drug properties. The aim of the present study encourages the use of spices as alternative or supplementary medicine to reduce the burden of high cost, side effects and progressively increasing drug resistance of pathogens. In present study methanol and chloroform extracts of Curcuma longa (fresh and dry) were prepared from the rhizome. The antimicrobial properties of both of the extract were studied by testing the antibacterial as well as antifungal activity. The antibacterial test was done by agar well diffusion method against bacterial species such as Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Protease vulgaris, Pseudomonas aeruginosa, Bacillus cereus, Bacillus megatarium, Bacillus subtilis and fungal species such as Aspergillus niger. The zone of inhibition of the extract were determined and compared with the standard drug streptomycin to know the efficiency. The methanol extract of rhizome was found to be more effective when compared with other solvent like chloroform. The phytochemical analysis of both of the extract revealed the presence of alkaloids, flavonoids, terpenoids, tannins, saponin and steroids.
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