在扩大工业企业活动中评估炭疽土栖动物境内感染风险的当前问题

L. Lukhnova, U. Izbanova, Toktasyn Erubaev, T. Meka-Mechenko, S. Sharipova, Erlan Sansyzbayev, Altyn Rysbekova, N. Turebekov, V. Sushchikh, Gulbostan Kim, A. Abiyeva, Nursultan Kunzhan, Galymzhan Tazhibaev
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Complete elimination of the infection is not possible due to the existence of soil-dwelling of anthrax. The article is devoted to assessing the risk of infection of people and animals on the territory of the soil-dwelling of anthrax and the possibility of reducing the sanitary protection zone (SPZ) in connection with the development of industrial enterprises. Aim. Laboratory-diagnostic analyses of samples of soil, groundwater, risk assessment of infection of people and animals on-site soil-dwelling of anthrax for the scientific substantiation of safe reduction of the SPZ soil-dwelling of the anthrax, which is located at an industrial facility. Methods. Samples and groundwater collection was carried out in accordance with the guidelines. The properties of the strains isolated from the samples were studied in accordance with generally accepted methods. To achieve this aim, the following methods were used to study samples for the presence of the causative agent of anthrax: bacteriological, biological, serological (fluorescence immunoassay, indirect hemagglutination test), genetic (PCR). The risk of infection was assessed on the territory of the soil dwelling (Aktobe region, Khromtau district, Khromtau) of anthrax using data on the epizootic and epidemic situation of anthrax. Results and discussions. Comprehensive studies of soil, surface and groundwater have been carried out. In bacteriological studies of soil samples in crops on the Hottinger's agar, single large, flat, matte-gray rough colonies with uneven edges and fringed processes were found. It was determined that all allocated isolates are typical soil microorganisms of Bacillus cereus involved in soil mineralization. There is no anthrax agent in the samples. We have assessed the risk of infection of people and animals in the territory of the soil dwelling of anthrax. 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引用次数: 0

摘要

根据最新的统计数据,在哈萨克斯坦共和国的经济结构中,注意到工业发展的增长率。16个区域中有14个区域提供了区域范围内工业生产的增长情况。采矿业提供了超过2.9%的就业机会和18%的经济总增加值。哈萨克斯坦的工业发展需要开发新的领土,由于存在炭疽(炭疽动物埋葬地)的土壤,这往往是困难的。哈萨克斯坦境内不适宜炭疽热。由于炭疽菌存在于土壤中,因此不可能完全消除感染。这篇文章致力于评估炭疽在土壤栖息区域的人和动物感染的风险,以及与工业企业发展有关的减少卫生保护区的可能性。的目标。对土壤、地下水样本进行实验室诊断分析,对现场炭疽菌土壤栖息地的人和动物感染风险进行评估,以科学地证实安全减少炭疽菌在工业设施的特别隔离区土壤栖息地。方法。样本和地下水的收集是按照准则进行的。按照普遍接受的方法对从样品中分离出的菌株的性质进行了研究。为了达到这一目的,采用以下方法研究炭疽病原体的存在:细菌学、生物学、血清学(荧光免疫测定、间接血凝试验)、遗传学(PCR)。利用炭疽动物流行病和流行情况数据,在炭疽土壤居住地(Aktobe地区,Khromtau地区,Khromtau)境内评估了感染风险。结果和讨论。开展了土壤、地表水和地下水综合研究。在Hottinger琼脂上对作物土壤样品进行细菌学研究时,发现了单个大的,扁平的,哑光灰色的粗糙菌落,边缘不均匀,边缘呈条纹状。结果表明,所分配的分离株均为蜡样芽孢杆菌参与土壤矿化的典型土壤微生物。样本中没有炭疽菌。我们已经评估了炭疽病土壤居住地境内的人和动物感染的风险。经确定,在阿克托别地区Khromtau地区的一个土壤居民点境内,易感动物和人感染炭疽的风险很低,该居民点位于一个工业设施的境内。结论。为解决炭疽菌土栖区安全缩小以扩大工业企业活动的问题,建议对土壤中心区域进行为期三年的系统微生物监测,采集至少200份土壤、地下水和地下水样本,然后进行炭疽杆菌存在情况调查和感染病原体的人、动物感染风险评估。关键词:炭疽;土栖;感染风险;
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Current questions of assessing the risk of infection in the territory of the soil-dwelling of anthrax for expanding the activities of industrial enterprises
In the structure of the economy of the Republic of Kazakhstan (RK) according to the latest statistics, the growth rate of industrial development is noted. Growth of industrial production in the regional context was provided in 14 regions out of 16. The mining sector provides more than 2.9% of employment and 18% of gross value added in the economy. The development of industry of Kazakhstan requires the development of new territories, which is often difficult due to the presence of soil-dwelling of anthrax (anthrax animal burial sites). The territory of Kazakhstan is unfavorable for anthrax. Complete elimination of the infection is not possible due to the existence of soil-dwelling of anthrax. The article is devoted to assessing the risk of infection of people and animals on the territory of the soil-dwelling of anthrax and the possibility of reducing the sanitary protection zone (SPZ) in connection with the development of industrial enterprises. Aim. Laboratory-diagnostic analyses of samples of soil, groundwater, risk assessment of infection of people and animals on-site soil-dwelling of anthrax for the scientific substantiation of safe reduction of the SPZ soil-dwelling of the anthrax, which is located at an industrial facility. Methods. Samples and groundwater collection was carried out in accordance with the guidelines. The properties of the strains isolated from the samples were studied in accordance with generally accepted methods. To achieve this aim, the following methods were used to study samples for the presence of the causative agent of anthrax: bacteriological, biological, serological (fluorescence immunoassay, indirect hemagglutination test), genetic (PCR). The risk of infection was assessed on the territory of the soil dwelling (Aktobe region, Khromtau district, Khromtau) of anthrax using data on the epizootic and epidemic situation of anthrax. Results and discussions. Comprehensive studies of soil, surface and groundwater have been carried out. In bacteriological studies of soil samples in crops on the Hottinger's agar, single large, flat, matte-gray rough colonies with uneven edges and fringed processes were found. It was determined that all allocated isolates are typical soil microorganisms of Bacillus cereus involved in soil mineralization. There is no anthrax agent in the samples. We have assessed the risk of infection of people and animals in the territory of the soil dwelling of anthrax. It was determined that there is a low risk of infection of susceptible animals and people with anthrax on the territory of a soil-dwelling located in the Khromtau district, Aktobe region on the territory of an industrial facility. Conclusions. To solve the problem of safe reduction of the SPZ of the soil-dwelling of anthrax in order to expand the activities of an industrial enterprise, it is recommended to conduct a systematic microbiological monitoring of the soil center territory for three years by taking at least 200 soil, groundwater and groundwater samples, followed by an investigation of the presence of the bacillus anthracis and risk assessment of infection of humans and animals with the causative agent of infection. Keywords: anthrax, soil-dwelling, risk of infection, sanitary protection zone.
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