用离体培养技术诱导山楂愈伤组织再生不定芽

M. Ibrahim, M. Z. Sabti, Shiamaa H. Mussa
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摘要

结果表明,在MS培养基中添加1.4 mg L-1 NAA和0.1 mg L-1 BA,愈伤组织诱导率达到96.67%,显著高于其他生长调节剂组合。结果表明,MS培养基中添加9.5 mg L-1 BA和0.2 mg L-1 NAA,培养12周后,小绒球愈伤组织间接不定芽形成的响应率最高,每100 mg愈伤组织再生数达到96.67%,每100 mg愈伤组织再生10个芽,茎长达到3.00 cm。在NAA浓度为1.0 mg L-1、BA浓度为0.1 mg L-1的MS培养基上培养,种植8周后可生根。
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Regeneration of Adventitious Shoots from Induced Callus of Lantana Camara L. Plant by in Vitro Culture Technique
The results showed that the MS medium supplemented with a concentration of 1.4 mg L-1 of NAA and 0.1 mg L-1 BA resulted in the highest significant value in the percentage of response to callus induction of 96.67% compared with the other combinations of growth regulators. The results showed that a combination of 9.5 mg L-1 BA and 0.2 mg L-1 NAA which was added to the MS medium recorded the highest percentage of response to indirect adventitious shoot formation from callus of Lantana Camara, several shoots per 100 mg callus and shoot length reached 96.67%, 10 shoots per 100 mg callus and 3.00 cm, respectively, after 12 weeks of culturing. The adventitious shoots of indirect organogenesis of Lantana Camara were rooted when cultured on the MS medium supplemented with a concentration of 1.0 mg L-1 of NAA and 0.1 mg L-1 BA after 8 weeks of planting.
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