利用酿酒酵母和米根霉生物量对香橼河水样中铬(VI)重金属的生物吸附

Suci Rizki Nurul Aeni, Ni'matul Murtafi'ah, Handarini Handarini
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摘要

2018年,西塔鲁姆河被认为是世界上最脏的河流,其特点是一个盆地毗邻几个纺织和电镀工业。六价铬金属(Cr6+)是一种有毒、致癌的重金属,存在于这些工业的废物中。此外,生物吸附剂(包括酿酒酵母菌和米根霉)的生物吸附是处理重金属污染水的一种替代方法。因此,本研究旨在利用S. cerevisiae和R. oryzae生物质在不同的生物质浓度和搅拌速度下,在Nanjung和Pangauban两个地点吸附Citarum河水中的Cr6 +重金属。在本研究中,以0.25、0.5、0.75 g r.m oryzae悬液以及250、500、750µS. cerevisiae作为吸附剂,分别在250、750、1500 rpm转速下旋转。随后,用紫外-可见分光光度计在525 nm波长处测量Cr6 +金属含量,并根据标准曲线计算。结果表明,酿酒酵母和米曲霉能够降低香橼河水中Cr6+的含量。当吸附剂浓度最高时,在1500rpm转速下,酿酒酵母(S. cerevisiae)的吸附剂浓度为750µ,稻瘟病菌(R. oryzae)的吸附剂浓度为0.75 g。酿酒葡萄球菌和米曲霉作为生物吸附剂在Citarum河重金属污染的原位修复中具有很大的潜力。
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Chromium(VI) Heavy Metal Biosorption in Citarum River Water Sample Using Saccharomyces cerevisiae and Rhizopus oryzae Biomass
The Citarum River was regarded as the World’s dirtiest river in 2018, characterized by a Basin situated adjacent to several textile and electroplating industries. Hexavalent chromium metal (Cr6+) is a toxic, carcinogenic heavy metal found in the wastes of these industries. Furthermore, biosorption with biological adsorbents, including Saccharomyces cerevisiae and Rhizopus oryzae, is an alternative method for treating water polluted with heavy metals. This study therefore aims to adsorb Cr6 + heavy metals from Citarum River water using S. cerevisiae and R. oryzae biomass in two locations: Nanjung and Pangauban, with various biomass concentrations and stirring speeds. For this study, 0.25, 0.5, and 0.75 g of R. oryzae suspension, as well as 250, 500, and 750 µ of S. cerevisiae were used as adsorbent, while rotated at speeds of 250 rpm, 750 rpm and 1500 rpm. Subsequently, the Cr6 + metal content was measured using a UV-Vis spectrophotometer at a wavelength of 525 nm, and calculated based on a standard curve. The results showed S. cerevisiae and R. oryzae are able to reduce the levels of Cr6+ in Citarum river water. The most reduction was obtained with the highest concentration of the adsorbents, 750 µ for S. cerevisiae and 0.75 g for R. oryzae, at the speed of 1500 rpm. S. cerevisiae and R. oryzae have great potential as biosorbents for the in situ remediation of Citarum River contaminated with heavy metals.
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