Pub Date : 2023-08-30DOI: 10.31258/biogenesis.19.2.101-117
Yunita Kesia S, I. Mahadi, S. Wulandari
Terasi memiliki warna asli coklat kehitaman yang berpengaruh terhadap daya tarik konsumen. Secara umum bahan pewarna yang sering digunakan terbagi atas pewarna alami dan sintetis (buatan). Pewarna sintetis lebih mudah ditemukan dipasaran dengan harga yang relatif lebih murah dibandingkan pewarna alami. Namun banyak sekali produsen yang menyalahgunakan pemakaian pewarna. Oleh karena itu dilakukan penelitian ini untuk mengetahui pengaruh limbah kulit buah manggis, kulit buah naga dan karmoisin terhadap kualitas terasi udang rebon (Acetes erythraeus) serta menghasilkan rancangan modul sebagai bahan ajar pada pembelajaran biologi kelas XII SMA. Penelitian ini terdiri dari dua tahap yaitu eksperimen dan perancangan modul pada bulan April-Mei 2021. Eksperimen dengan desain Rancangan Acak Lengkap (RAL) dan menggunakan 4 perlakuan dengan 3 kali ulangan. Perlakuan yang diberikan berupa perbedaan pewarna, pewarna alami (kulit buah manggis dan kulit buah naga) dan pewarna sintetis (karmoisin). Adapun perlakuan: P0(perlakuan tanpa pewarna), P1 (ekstrak pewarna kulit buah manggis), P2 (ekstrak pewarna kulit buah naga), dan P3 (pewarna karmoisin). Parameter yang diukur meliputi kadar protein, kadar lemak, kadar pH, kadar air, dan uji organoleptik. Hasil penelitian menunjukkan bahwa limbah kulit buah berpengaruh sangat nyata terhadap kualitas terasi udang rebon. Perlakuan yang terbaik adalah P1 (ekstrak pewarna kulit buah manggis) dengan kadar protein sebesar 20,15%, kadar lemak 0,08%, kadar pH 6,8, kadar air 23,97%, nilai uji kenampakan 8,9, nilai uji bau 8,6, nilai uji rasa 8,6, nilai uji tekstur 8,5 dan nilai hedonik 8,6. Hasil eksperimen digunakan untuk modul sebagai bahan ajar pada materi bioteknologi konvensional pada pembelajaran Biologi di SMA kelas XII.
{"title":"PENGARUH JENIS PEWARNAAN TERHADAP KUALITAS TERASI UDANG REBON(Acetes erythraeus) SEBAGAI RANCANGAN MODUL PADA MATERI BIOTEKNOLOGI SMA KELAS XII","authors":"Yunita Kesia S, I. Mahadi, S. Wulandari","doi":"10.31258/biogenesis.19.2.101-117","DOIUrl":"https://doi.org/10.31258/biogenesis.19.2.101-117","url":null,"abstract":"Terasi memiliki warna asli coklat kehitaman yang berpengaruh terhadap daya tarik konsumen. Secara umum bahan pewarna yang sering digunakan terbagi atas pewarna alami dan sintetis (buatan). Pewarna sintetis lebih mudah ditemukan dipasaran dengan harga yang relatif lebih murah dibandingkan pewarna alami. Namun banyak sekali produsen yang menyalahgunakan pemakaian pewarna. Oleh karena itu dilakukan penelitian ini untuk mengetahui pengaruh limbah kulit buah manggis, kulit buah naga dan karmoisin terhadap kualitas terasi udang rebon (Acetes erythraeus) serta menghasilkan rancangan modul sebagai bahan ajar pada pembelajaran biologi kelas XII SMA. Penelitian ini terdiri dari dua tahap yaitu eksperimen dan perancangan modul pada bulan April-Mei 2021. Eksperimen dengan desain Rancangan Acak Lengkap (RAL) dan menggunakan 4 perlakuan dengan 3 kali ulangan. Perlakuan yang diberikan berupa perbedaan pewarna, pewarna alami (kulit buah manggis dan kulit buah naga) dan pewarna sintetis (karmoisin). Adapun perlakuan: P0(perlakuan tanpa pewarna), P1 (ekstrak pewarna kulit buah manggis), P2 (ekstrak pewarna kulit buah naga), dan P3 (pewarna karmoisin). Parameter yang diukur meliputi kadar protein, kadar lemak, kadar pH, kadar air, dan uji organoleptik. Hasil penelitian menunjukkan bahwa limbah kulit buah berpengaruh sangat nyata terhadap kualitas terasi udang rebon. Perlakuan yang terbaik adalah P1 (ekstrak pewarna kulit buah manggis) dengan kadar protein sebesar 20,15%, kadar lemak 0,08%, kadar pH 6,8, kadar air 23,97%, nilai uji kenampakan 8,9, nilai uji bau 8,6, nilai uji rasa 8,6, nilai uji tekstur 8,5 dan nilai hedonik 8,6. Hasil eksperimen digunakan untuk modul sebagai bahan ajar pada materi bioteknologi konvensional pada pembelajaran Biologi di SMA kelas XII.","PeriodicalId":8911,"journal":{"name":"Biogenesis: Jurnal Ilmiah Biologi","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84426058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-30DOI: 10.31258/biogenesis.19.2.135-153
Dewi Asih Wulandari, M. L, Fitra Suzanti
E-LKPD merupakan sumber belajar versi elektronik yang dapat dibuka pada layar laptop ataupun handphone yang dapat digunakan siswa untuk mempelajari materi keanekaragaman hayati di kelas X. Tujuan dari penelitian ini adalah untuk menghasilkan E-LKPD berbasis Guided Inquiry pada materi keanekaragaman hayati untuk siswa kelas X SMA yang berkualitas. Penelitian ini dilaksanakan di Kampus Pendidikan Biologi FKIP Universitas Riau, SMAN 1 Pekanbaru, dan SMAN 8 Pekanbaru. Jenis penelitian ini adalah penelitian pengembangan dengan menggunakan model ADDIE. Pada penelitian ini hanya dilakukan hingga tahap Pengembangan. Instrumen pengumpulan data yang digunakan adalah lembar validasi dan angket respon. Pengumpulan data dilakukan dengan melakukan validasi terhadap E-LKPD yang dikembangkan, uji coba terbatas tahap I dan II. Teknik analisis data yang digunakan adalah analisis deskriptif kuantitatif. Hasil penelitian menunjukkan bahwa hasil validasi E-LKPD pada keseluruhan aspek mendapatkan nilai rata-rata sebesar 3.69 dengan kategori sangat valid, hasil uji coba terbatas tahap I dan uji coba terbatas tahap II pada E-LKPD mendapatkan nilai rata-rata uji coba terbatas tahap I sebesar 3.74 dengan kategori sangat baik, dan nilai rata-rata uji coba terbatas tahap II sebesar 3.77 dengan kategori sangat baik. Berdasarkan hasil penelitian dapat disimpulkan bahwa E-LKPD berbasis Guided Inquiry pada materi keanekaragaman hayati untuk siswa kelas X SMA yang telah dikembangkan memiliki kualitas yang sangat baik dengan nilai kualitas sebesar 3.73.
{"title":"PENGEMBANGAN E-LKPD BERBASIS GUIDED INQUIRY PADA MATERI KEANEKARAGAMAN HAYATI UNTUK SISWA KELAS X SMA","authors":"Dewi Asih Wulandari, M. L, Fitra Suzanti","doi":"10.31258/biogenesis.19.2.135-153","DOIUrl":"https://doi.org/10.31258/biogenesis.19.2.135-153","url":null,"abstract":"E-LKPD merupakan sumber belajar versi elektronik yang dapat dibuka pada layar laptop ataupun handphone yang dapat digunakan siswa untuk mempelajari materi keanekaragaman hayati di kelas X. Tujuan dari penelitian ini adalah untuk menghasilkan E-LKPD berbasis Guided Inquiry pada materi keanekaragaman hayati untuk siswa kelas X SMA yang berkualitas. Penelitian ini dilaksanakan di Kampus Pendidikan Biologi FKIP Universitas Riau, SMAN 1 Pekanbaru, dan SMAN 8 Pekanbaru. Jenis penelitian ini adalah penelitian pengembangan dengan menggunakan model ADDIE. Pada penelitian ini hanya dilakukan hingga tahap Pengembangan. Instrumen pengumpulan data yang digunakan adalah lembar validasi dan angket respon. Pengumpulan data dilakukan dengan melakukan validasi terhadap E-LKPD yang dikembangkan, uji coba terbatas tahap I dan II. Teknik analisis data yang digunakan adalah analisis deskriptif kuantitatif. Hasil penelitian menunjukkan bahwa hasil validasi E-LKPD pada keseluruhan aspek mendapatkan nilai rata-rata sebesar 3.69 dengan kategori sangat valid, hasil uji coba terbatas tahap I dan uji coba terbatas tahap II pada E-LKPD mendapatkan nilai rata-rata uji coba terbatas tahap I sebesar 3.74 dengan kategori sangat baik, dan nilai rata-rata uji coba terbatas tahap II sebesar 3.77 dengan kategori sangat baik. Berdasarkan hasil penelitian dapat disimpulkan bahwa E-LKPD berbasis Guided Inquiry pada materi keanekaragaman hayati untuk siswa kelas X SMA yang telah dikembangkan memiliki kualitas yang sangat baik dengan nilai kualitas sebesar 3.73.","PeriodicalId":8911,"journal":{"name":"Biogenesis: Jurnal Ilmiah Biologi","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87577054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-30DOI: 10.31258/biogenesis.19.2.118-134
Ria Mariati, M. L, Darmadi Darmadi
Penelitian ini bertujuan untuk menghasilkan modul elektronik berbasis Flip PDF Professional pada materi narkotika, psikotropika, dan zat adiktif di kelas XI SMA yang berkualitas. Penelitian ini dilaksanakan di Program Studi Pendidikan Biologi FKIP Universitas Riau, SMAN 1 Pekanbaru, dan SMAN Plus Provinsi Riau pada bulan Februari 2021–April 2022. Jenis penelitian ini adalah penelitian pengembangan (Research and Development) menggunakan model ADDIE, yang terdiri dari tahap analisis, desain, dan pengembangan. Tahap analisis yaitu menganalisis kurikulum, analisis karakteristik siswa, dan analisis materi pembelajaran. Tahap desain yaitu mendesain perangkat pembelajaran dan storyboard modul elektronik. Tahap pengembangan yaitu mengembangkan produk modul elektronik berbasis Flip PDF Professional, melakukan validasi dan uji coba terbatas tahap I dan II terhadap modul elektronik. Instrumen pengumpul data yang digunakan adalah lembar validasi dan angket respon, terdiri dari aspek format dan kegrafisan, aspek isi/materi, aspek bahasa, dan aspek berbasis Flip PDF Professional. Data hasil validasi dan uji coba terbatas dianalisis menggunakan analisis deskriptif kuantitatif. Hasil penelitian menunjukkan bahwa validasi modul elektronik mendapatkan nilai rata-rata 3.74 dengan kategori sangat valid. Uji coba terbatas tahap I dan II mendapatkan nilai rata-rata 3.78 dengan kategori sangat baik. Kualitas modul elektronik mendapatkan nilai rata-rata 3.76 dengan kategori sangat baik. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa modul elektronik berbasis Flip PDF Professional pada materi narkotika, psikotropika, dan zat adiktif di kelas XI SMA yang telah dikembangkan memiliki kualitas sangat baik
{"title":"PENGEMBANGAN MODUL ELEKTRONIK BERBASIS FLIP PDF PROFESSIONAL PADA MATERI NARKOTIKA, PSIKOTROPIKA, DAN ZAT ADIKTIF DI KELAS XI SMA","authors":"Ria Mariati, M. L, Darmadi Darmadi","doi":"10.31258/biogenesis.19.2.118-134","DOIUrl":"https://doi.org/10.31258/biogenesis.19.2.118-134","url":null,"abstract":"Penelitian ini bertujuan untuk menghasilkan modul elektronik berbasis Flip PDF Professional pada materi narkotika, psikotropika, dan zat adiktif di kelas XI SMA yang berkualitas. Penelitian ini dilaksanakan di Program Studi Pendidikan Biologi FKIP Universitas Riau, SMAN 1 Pekanbaru, dan SMAN Plus Provinsi Riau pada bulan Februari 2021–April 2022. Jenis penelitian ini adalah penelitian pengembangan (Research and Development) menggunakan model ADDIE, yang terdiri dari tahap analisis, desain, dan pengembangan. Tahap analisis yaitu menganalisis kurikulum, analisis karakteristik siswa, dan analisis materi pembelajaran. Tahap desain yaitu mendesain perangkat pembelajaran dan storyboard modul elektronik. Tahap pengembangan yaitu mengembangkan produk modul elektronik berbasis Flip PDF Professional, melakukan validasi dan uji coba terbatas tahap I dan II terhadap modul elektronik. Instrumen pengumpul data yang digunakan adalah lembar validasi dan angket respon, terdiri dari aspek format dan kegrafisan, aspek isi/materi, aspek bahasa, dan aspek berbasis Flip PDF Professional. Data hasil validasi dan uji coba terbatas dianalisis menggunakan analisis deskriptif kuantitatif. Hasil penelitian menunjukkan bahwa validasi modul elektronik mendapatkan nilai rata-rata 3.74 dengan kategori sangat valid. Uji coba terbatas tahap I dan II mendapatkan nilai rata-rata 3.78 dengan kategori sangat baik. Kualitas modul elektronik mendapatkan nilai rata-rata 3.76 dengan kategori sangat baik. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa modul elektronik berbasis Flip PDF Professional pada materi narkotika, psikotropika, dan zat adiktif di kelas XI SMA yang telah dikembangkan memiliki kualitas sangat baik","PeriodicalId":8911,"journal":{"name":"Biogenesis: Jurnal Ilmiah Biologi","volume":"10 3-4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75498726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bakteri Asam Laktat (BAL) termasuk dalam salah satu mikroorganisme menguntungkan yang ditemukan didalam tempe. Nutrisi yang terdapat didalam tempe membantu BAL menghasilkan metabolit aktif sehingga senyawa tersebut dapat berperan dalam menjaga kesehatan saluran pencernaan manusia. BAL mampu menekan pertumbuhan bakteri patogen, dapat meningkatkan aroma, cita rasa dan warna pada tempe. Pembuatan tempe masih dilakukan secara tradisional dan dilakukan secara turun temurun dengan cara yang berbeda. Hal ini memungkinkan tumbuhnya perbedaan jumlah BAL pada setiap tempe. Tujuan penelitian yaitu untuk mengetahui perbandingan jumlah total BAL pada dua sampel tempe yang berbeda. Sampel yang digunakan adalah tempe D dan E yang berasal dari daerah Bekasi dan dari pengrajin yang berbeda. Metode yang digunakan adalah pengenceran bertingkat dengan spread plate. Penghitungan total BAL dilakukan pada cawan yang berisi 25-250 koloni dengan menggunakan rumus Total Plate Count (TPC). Identifikasi dilakukan secara makroskopis dan mikroskopis. Hasil penelitian menunjukkan bahwa sampel tempe D dan E memiliki total BAL berbeda yaitu berturut-turut 5,6x106 dan 1,2x107 CFU/g. Sampel tempe E memiliki total BAL tertinggi daripada sampel tempe D. Kedua sampel tempe memenuhi syarat standar bakteri probiotik yaitu 106 hingga 107 CFU/g. Secara makroskopis, kedua sampel tempe memiliki BAL dengan warna koloni putih susu, bulat, tepian rata dan elevasi cembung. Sedangkan, secara mikroskopis BAL bersifat Gram positif dengan bentuk sel yang beragam.
{"title":"PERBANDINGAN JUMLAH TOTAL BAKTERI ASAM LAKTAT DARI DUA SAMPEL TEMPE BERBEDA DI BEKASI","authors":"Qurrota Aýun, Karina Sheilla Nazua, Isnain Ardiani, Imelda Pujiharti","doi":"10.31258/biogenesis.19.2.164-173","DOIUrl":"https://doi.org/10.31258/biogenesis.19.2.164-173","url":null,"abstract":"Bakteri Asam Laktat (BAL) termasuk dalam salah satu mikroorganisme menguntungkan yang ditemukan didalam tempe. Nutrisi yang terdapat didalam tempe membantu BAL menghasilkan metabolit aktif sehingga senyawa tersebut dapat berperan dalam menjaga kesehatan saluran pencernaan manusia. BAL mampu menekan pertumbuhan bakteri patogen, dapat meningkatkan aroma, cita rasa dan warna pada tempe. Pembuatan tempe masih dilakukan secara tradisional dan dilakukan secara turun temurun dengan cara yang berbeda. Hal ini memungkinkan tumbuhnya perbedaan jumlah BAL pada setiap tempe. Tujuan penelitian yaitu untuk mengetahui perbandingan jumlah total BAL pada dua sampel tempe yang berbeda. Sampel yang digunakan adalah tempe D dan E yang berasal dari daerah Bekasi dan dari pengrajin yang berbeda. Metode yang digunakan adalah pengenceran bertingkat dengan spread plate. Penghitungan total BAL dilakukan pada cawan yang berisi 25-250 koloni dengan menggunakan rumus Total Plate Count (TPC). Identifikasi dilakukan secara makroskopis dan mikroskopis. Hasil penelitian menunjukkan bahwa sampel tempe D dan E memiliki total BAL berbeda yaitu berturut-turut 5,6x106 dan 1,2x107 CFU/g. Sampel tempe E memiliki total BAL tertinggi daripada sampel tempe D. Kedua sampel tempe memenuhi syarat standar bakteri probiotik yaitu 106 hingga 107 CFU/g. Secara makroskopis, kedua sampel tempe memiliki BAL dengan warna koloni putih susu, bulat, tepian rata dan elevasi cembung. Sedangkan, secara mikroskopis BAL bersifat Gram positif dengan bentuk sel yang beragam.","PeriodicalId":8911,"journal":{"name":"Biogenesis: Jurnal Ilmiah Biologi","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88889564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-30DOI: 10.31258/biogenesis.19.2.84-100
D. Anggraini, Ign. Sudaryadi
Paparan sinar ultraviolet (UV) dalam jangka waktu panjang dapat menimbulkan stress oksidatif akibat terbentuknya Reactive Oxygen Species (ROS) secara berlebih. Salah satu upaya yang dapat dilakukan untuk menangkal ROS adalah dengan mengonsumsi makanan kaya antioksidan seperti sayur, buah, dan alga. Eucheuma sp. merupakan makroalga yang berpotensi sebagai sumber antioksidan karena mengandung beragam senyawa bioaktif. Lalat buah (Drosophila melanogaster) merupakan organisme model untuk mempelajari berbagai disiplin ilmu karena memiliki anatomi, fisiologi, dan sistem organ yang sangat kompleks seperti manusia. Penelitian ini bertujuan untuk mengetahui pengaruh radiasi sinar UV terhadap sintasan lalat buah pada tiap fase kehidupannya, mengidentifikasi senyawa bioaktif Eucheuma sp. dengan metode Gas Chromatography/Mass Spectrometry (GC/MS) pelarut metanol, serta mengetahui kemampuan proteksi yang dihasilkan pakan ekstrak Eucheuma sp. dan pakan pisang terhadap sintasan lalat buah. Tiap kelompok lalat buah diberi perlakuan yang berbeda, yaitu perlakuan tanpa paparan dan dengan paparan sinar UV selama 2 jam dalam 3 hari, serta perlakuan pakan ekstrak Eucheuma sp. dan pakan standar pisang. Data dianalisis dengan uji One way Anova dan uji lanjutan Duncan (α = 0,05). Hasil penelitian menunjukkan bahwa radiasi sinar UV dapat mempengaruhi sintasan lalat buah pada fase larva, pupa, dan imago. Nilai kemampuan sintasan tiap perlakuan yaitu 24,9% (PPI), 5,9% (PPIUV), 2,7% (PRL), dan 4,0% (PRLUV).
{"title":"PENGARUH RADIASI SINAR ULTRAVIOLET DAN PEMBERIAN PAKAN EKSTRAK RUMPUT LAUT MERAH (Eucheuma sp.) TERHADAP SINTASAN LALAT BUAH (Drosophila melanogaster)","authors":"D. Anggraini, Ign. Sudaryadi","doi":"10.31258/biogenesis.19.2.84-100","DOIUrl":"https://doi.org/10.31258/biogenesis.19.2.84-100","url":null,"abstract":"Paparan sinar ultraviolet (UV) dalam jangka waktu panjang dapat menimbulkan stress oksidatif akibat terbentuknya Reactive Oxygen Species (ROS) secara berlebih. Salah satu upaya yang dapat dilakukan untuk menangkal ROS adalah dengan mengonsumsi makanan kaya antioksidan seperti sayur, buah, dan alga. Eucheuma sp. merupakan makroalga yang berpotensi sebagai sumber antioksidan karena mengandung beragam senyawa bioaktif. Lalat buah (Drosophila melanogaster) merupakan organisme model untuk mempelajari berbagai disiplin ilmu karena memiliki anatomi, fisiologi, dan sistem organ yang sangat kompleks seperti manusia. Penelitian ini bertujuan untuk mengetahui pengaruh radiasi sinar UV terhadap sintasan lalat buah pada tiap fase kehidupannya, mengidentifikasi senyawa bioaktif Eucheuma sp. dengan metode Gas Chromatography/Mass Spectrometry (GC/MS) pelarut metanol, serta mengetahui kemampuan proteksi yang dihasilkan pakan ekstrak Eucheuma sp. dan pakan pisang terhadap sintasan lalat buah. Tiap kelompok lalat buah diberi perlakuan yang berbeda, yaitu perlakuan tanpa paparan dan dengan paparan sinar UV selama 2 jam dalam 3 hari, serta perlakuan pakan ekstrak Eucheuma sp. dan pakan standar pisang. Data dianalisis dengan uji One way Anova dan uji lanjutan Duncan (α = 0,05). Hasil penelitian menunjukkan bahwa radiasi sinar UV dapat mempengaruhi sintasan lalat buah pada fase larva, pupa, dan imago. Nilai kemampuan sintasan tiap perlakuan yaitu 24,9% (PPI), 5,9% (PPIUV), 2,7% (PRL), dan 4,0% (PRLUV). ","PeriodicalId":8911,"journal":{"name":"Biogenesis: Jurnal Ilmiah Biologi","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83621089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lemongrass (Cymbopogon nardus (L.) Rendle) is one of plants with many benefits, which the main compound is able to inhibit bacterial activity. Utilizing citronella essential oil in an emulgel dose form can help treat Staphylococcus aureus. This study aims to determine the antibacterial effect of essential oil concentrations of citronella emulgel preparations at concentrations of 10%, 20%, 30%, and negative control against S. aureus. The used method was agar diffusion by means of wells. Data were analyzed by One-Way ANOVA test and Post Hoc test in SPSS ver. 26. Lemongrass essential oil emulgel was proven to be able to inhibit the growth of S. aureus as evidenced by the inhibition zone formed. The diameter of the inhibition zones for the 10%, 20%, 30% concentrations were 5.367 mm, 7.867 mm, and 11.833 mm, respectively. While the emulgel base as a control was 0 mm. The results of the ANOVA test showed a significant value of 0.000<0.05, so there was a significant difference between the inhibition zone and the 95% confidence level. Based on the Post Hoc test, the inhibition of the three groups of emulgel formulations of citronella essential oil was significantly different from the control group. The three emulgel concentration groups proved to be significantly different, in which citronella essential oil emulgel with a concentration of 30% had the best antibacterial activity. This finding revealed the optimum concentration for the emulsion of lemongrass essential oil to promote antibacterial activity.
{"title":"Antibacterial activity of emulgel essential oil from scented lemongrass (Cymbopogon nardus (L.) Rendle) against Staphylococcus aureus ATCC-29213","authors":"None Fianicha Shalihah, Alanindra Saputra, Yasir Sidiq","doi":"10.24252/bio.v11i1.35836","DOIUrl":"https://doi.org/10.24252/bio.v11i1.35836","url":null,"abstract":"Lemongrass (Cymbopogon nardus (L.) Rendle) is one of plants with many benefits, which the main compound is able to inhibit bacterial activity. Utilizing citronella essential oil in an emulgel dose form can help treat Staphylococcus aureus. This study aims to determine the antibacterial effect of essential oil concentrations of citronella emulgel preparations at concentrations of 10%, 20%, 30%, and negative control against S. aureus. The used method was agar diffusion by means of wells. Data were analyzed by One-Way ANOVA test and Post Hoc test in SPSS ver. 26. Lemongrass essential oil emulgel was proven to be able to inhibit the growth of S. aureus as evidenced by the inhibition zone formed. The diameter of the inhibition zones for the 10%, 20%, 30% concentrations were 5.367 mm, 7.867 mm, and 11.833 mm, respectively. While the emulgel base as a control was 0 mm. The results of the ANOVA test showed a significant value of 0.000<0.05, so there was a significant difference between the inhibition zone and the 95% confidence level. Based on the Post Hoc test, the inhibition of the three groups of emulgel formulations of citronella essential oil was significantly different from the control group. The three emulgel concentration groups proved to be significantly different, in which citronella essential oil emulgel with a concentration of 30% had the best antibacterial activity. This finding revealed the optimum concentration for the emulsion of lemongrass essential oil to promote antibacterial activity.","PeriodicalId":8911,"journal":{"name":"Biogenesis: Jurnal Ilmiah Biologi","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136163885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.24252/bio.v11i1.32991
None Reza Anindita, None Maulin Inggraini
The prevalence of helminthiasis in West Java is 13.9%, but there is no data on STH helminthiasis on school-age children in the final garbage dump (FGD), Bantar Gebang, East Bekasi, Indonesia. Therefore, it is necessary to monitor STH helminthiasis on school-age children living around FGD. This study aims to determine the description of helminthiasis on school-age children who live in the FGD Bantar Gebang, Indonesia. This type of research is descriptive quantitative with a cross-sectional design. The sample in this study consisted of 79 stool children aged 5-9 years, 5 g of fly vectors from wet, dry, and soil were taken from 36 locations around the FGD. STH examination on stool, flies, and soil samples was carried out using the native, Harada Mori, flotation, and sedimentation methods. All data were analyzed using a descriptive approach to determine the percentage of the incidence of STH. The results of this study, among others, from 79 children there were six (7.60%) children infected with STH with the genus or species Ascaris lumbricoides, hookworm, and Trichuris trichiura. As for the 18 points of soil extraction, there were 12 (66.7%) positive points containing hookworm larvae, while the flies found were more dominant in Musca domestica than Chrysomya sp. with worm parasites attached to the fly's body are Ascaris lumbricoides, Diphyllobothrium latum. Enterobius vermicularis, hookworm, Hymenolepis sp., Schistosoma sp., Trichuris trichiura. The conclusion of this study is percentage incidence of STH helminthiasis on school-age children living in FGD Bantar Gebang is 7.60% which is in the low category.
{"title":"Monitoring of soil transmitted helminth (STH) Helminthiasis on school-age children in final garbage dump, Bantar Gebang, East Bekasi","authors":"None Reza Anindita, None Maulin Inggraini","doi":"10.24252/bio.v11i1.32991","DOIUrl":"https://doi.org/10.24252/bio.v11i1.32991","url":null,"abstract":"The prevalence of helminthiasis in West Java is 13.9%, but there is no data on STH helminthiasis on school-age children in the final garbage dump (FGD), Bantar Gebang, East Bekasi, Indonesia. Therefore, it is necessary to monitor STH helminthiasis on school-age children living around FGD. This study aims to determine the description of helminthiasis on school-age children who live in the FGD Bantar Gebang, Indonesia. This type of research is descriptive quantitative with a cross-sectional design. The sample in this study consisted of 79 stool children aged 5-9 years, 5 g of fly vectors from wet, dry, and soil were taken from 36 locations around the FGD. STH examination on stool, flies, and soil samples was carried out using the native, Harada Mori, flotation, and sedimentation methods. All data were analyzed using a descriptive approach to determine the percentage of the incidence of STH. The results of this study, among others, from 79 children there were six (7.60%) children infected with STH with the genus or species Ascaris lumbricoides, hookworm, and Trichuris trichiura. As for the 18 points of soil extraction, there were 12 (66.7%) positive points containing hookworm larvae, while the flies found were more dominant in Musca domestica than Chrysomya sp. with worm parasites attached to the fly's body are Ascaris lumbricoides, Diphyllobothrium latum. Enterobius vermicularis, hookworm, Hymenolepis sp., Schistosoma sp., Trichuris trichiura. The conclusion of this study is percentage incidence of STH helminthiasis on school-age children living in FGD Bantar Gebang is 7.60% which is in the low category.","PeriodicalId":8911,"journal":{"name":"Biogenesis: Jurnal Ilmiah Biologi","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136367121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.24252/bio.v11i1.36824
Anamika Gupta, Deeksha Singh, Rajiv Ranjan
Plants serve as a boundless source of raw materials for pharmaceuticals and have been used as an important source of medicine for several years. Identifying natural compounds from medicinal plants is useful for the discovery of novel therapeutic agents. Tylophora indica is perennial climber which possess many medicinal properties. Although it also contains many bioactive compounds which have role in various biological activity such as anti-inflammatory activity, anti-oxidant activity, and anti-cancer activity. The present study aimed to identify the bioactive compounds from methanolic and hexane extract of the leaves, stems, and roots of T. indica using GC-MS analysis. GC-MS analysis was performed by using standard protocols. Willey and NIST libraries were used in the identification of components, and their retention indices were compared. The GC-MS analysis revealed the presence of various prevailing compounds like Levomenthol, 2-Butanone, 3,3-dimethyl-1-(methylsulfonyl)-,O[(methylamino)carbonyl] oxime, Squalene, 5–methyl–2–(1–methylethyl)–(1α,2β,5β)– cyclohexanol, Oleic acid and 2-propyloxirane etc. These identified compounds play a vital role in various biological activities which were confirmed by Dr. Duke’s phytochemical and ethnobotanical databases (https://phytochem.nal.usda.gov/). The findings of this study demonstrate that T. indica is a rich source of numerous bioactive compounds that can be useful in halting the progression of several disorders.
{"title":"GC-MS analysis of bioactive compounds present in leaves, stem, and roots extract of Tylophora indica (Burm.fil.) Merr.","authors":"Anamika Gupta, Deeksha Singh, Rajiv Ranjan","doi":"10.24252/bio.v11i1.36824","DOIUrl":"https://doi.org/10.24252/bio.v11i1.36824","url":null,"abstract":"Plants serve as a boundless source of raw materials for pharmaceuticals and have been used as an important source of medicine for several years. Identifying natural compounds from medicinal plants is useful for the discovery of novel therapeutic agents. Tylophora indica is perennial climber which possess many medicinal properties. Although it also contains many bioactive compounds which have role in various biological activity such as anti-inflammatory activity, anti-oxidant activity, and anti-cancer activity. The present study aimed to identify the bioactive compounds from methanolic and hexane extract of the leaves, stems, and roots of T. indica using GC-MS analysis. GC-MS analysis was performed by using standard protocols. Willey and NIST libraries were used in the identification of components, and their retention indices were compared. The GC-MS analysis revealed the presence of various prevailing compounds like Levomenthol, 2-Butanone, 3,3-dimethyl-1-(methylsulfonyl)-,O[(methylamino)carbonyl] oxime, Squalene, 5–methyl–2–(1–methylethyl)–(1α,2β,5β)– cyclohexanol, Oleic acid and 2-propyloxirane etc. These identified compounds play a vital role in various biological activities which were confirmed by Dr. Duke’s phytochemical and ethnobotanical databases (https://phytochem.nal.usda.gov/). The findings of this study demonstrate that T. indica is a rich source of numerous bioactive compounds that can be useful in halting the progression of several disorders.","PeriodicalId":8911,"journal":{"name":"Biogenesis: Jurnal Ilmiah Biologi","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136367123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.24252/bio.v11i1.35632
Ferymon Mahulette, Esti Utarti, Tri Santi Kurnia
Nutmeg (Myristica fragrans Houtt) is commonly cultivated by people in the forests of Moluccas Islands. This plant grows well on relatively infertile soil types. This is presumably due to the presence of symbiotic microbes in the root of nutmeg. The research aimed to isolate, characterize and test the potential of Actinomycetes from rhizosphere of nutmeg. Soil sample were taken from the nutmeg forest in Ambon Island. The Actinomycetes isolation using humic acid vitamin, continued with yeast malt agar (YMA) media. The testing of antibacterial and antifungal activities using YMA media, while cellulolytic activity, phosphate solubilizing, and xylanolytic activity using carboxyl methyl cellulose, Picovskaya agar, and birchwood agar or oat spelt xylan agar. A total of 12 isolates of Actinomycetes were isolated and dominated by Streptomyces with various types of aerial mycelia. The substrate mycelium looks brown and cream, while the aerial mycelium looks white and gray. These isolates had the highest inhibitory power against Escherichia coli and Fusarium oxysporum with indexes of 16.5 mm and 16.0 mm, respectively. The other isolates have the ability of cellulolytic, phosphate solubilizing, and xylanolytic with indexes 3.26, 3.87, and 1.2, respectively. The Actinomycetes isolates that were found can be used as starter to improve the biofertilizer formula for nutmeg.
{"title":"Isolation and potency of Actinomycetes from rhizosphere of nutmeg (Myristica fragrans Houtt)","authors":"Ferymon Mahulette, Esti Utarti, Tri Santi Kurnia","doi":"10.24252/bio.v11i1.35632","DOIUrl":"https://doi.org/10.24252/bio.v11i1.35632","url":null,"abstract":"Nutmeg (Myristica fragrans Houtt) is commonly cultivated by people in the forests of Moluccas Islands. This plant grows well on relatively infertile soil types. This is presumably due to the presence of symbiotic microbes in the root of nutmeg. The research aimed to isolate, characterize and test the potential of Actinomycetes from rhizosphere of nutmeg. Soil sample were taken from the nutmeg forest in Ambon Island. The Actinomycetes isolation using humic acid vitamin, continued with yeast malt agar (YMA) media. The testing of antibacterial and antifungal activities using YMA media, while cellulolytic activity, phosphate solubilizing, and xylanolytic activity using carboxyl methyl cellulose, Picovskaya agar, and birchwood agar or oat spelt xylan agar. A total of 12 isolates of Actinomycetes were isolated and dominated by Streptomyces with various types of aerial mycelia. The substrate mycelium looks brown and cream, while the aerial mycelium looks white and gray. These isolates had the highest inhibitory power against Escherichia coli and Fusarium oxysporum with indexes of 16.5 mm and 16.0 mm, respectively. The other isolates have the ability of cellulolytic, phosphate solubilizing, and xylanolytic with indexes 3.26, 3.87, and 1.2, respectively. The Actinomycetes isolates that were found can be used as starter to improve the biofertilizer formula for nutmeg.","PeriodicalId":8911,"journal":{"name":"Biogenesis: Jurnal Ilmiah Biologi","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136367122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.24252/bio.v11i1.35297
Arifin Surya Dwipa Irsyam, Muhammad Rifqi Hariri, Muhammad Fabio Rayhan Kurniawan, Rina Ratnasih Irwanto
Plectranthus (Lamiaceae) is a popular ornamental plant all over the world. The study of the genus Plectranthus in Java was considered complete after the publication of Flora of Java Vol. II. Many authors, however, have reported the presence of numerous alien species on the island in recent years. The aim of this research is to provide current information on Plectranthus in Java, particularly the newly recorded alien species. The study was carried out using the free exploration method in the provinces of Banten, Jakarta, West Java, and East Java. We reported the first occurrence of P. verticillatus (L.f.) Druce in Java in this paper. The species is a succulent herb native to Southern Africa that has grown in popularity as an ornamental plant throughout the world. It is a newly discovered alien species to the Alien Flora of Java. The species appears to have escaped cultivation, with spontaneous populations found in Jatihandap and Cipadung, both in Bandung City. We also reported that P. verticillatus is the only member of Plectranthus found in Java in a recent taxonomic study. There is a description, photographs, botanical illustrations, and a brief discussion.
{"title":"Study on the Genus Plectranthus (Lamiaceae) in Java: P. verticillatus, a captivating new alien species","authors":"Arifin Surya Dwipa Irsyam, Muhammad Rifqi Hariri, Muhammad Fabio Rayhan Kurniawan, Rina Ratnasih Irwanto","doi":"10.24252/bio.v11i1.35297","DOIUrl":"https://doi.org/10.24252/bio.v11i1.35297","url":null,"abstract":"Plectranthus (Lamiaceae) is a popular ornamental plant all over the world. The study of the genus Plectranthus in Java was considered complete after the publication of Flora of Java Vol. II. Many authors, however, have reported the presence of numerous alien species on the island in recent years. The aim of this research is to provide current information on Plectranthus in Java, particularly the newly recorded alien species. The study was carried out using the free exploration method in the provinces of Banten, Jakarta, West Java, and East Java. We reported the first occurrence of P. verticillatus (L.f.) Druce in Java in this paper. The species is a succulent herb native to Southern Africa that has grown in popularity as an ornamental plant throughout the world. It is a newly discovered alien species to the Alien Flora of Java. The species appears to have escaped cultivation, with spontaneous populations found in Jatihandap and Cipadung, both in Bandung City. We also reported that P. verticillatus is the only member of Plectranthus found in Java in a recent taxonomic study. There is a description, photographs, botanical illustrations, and a brief discussion.","PeriodicalId":8911,"journal":{"name":"Biogenesis: Jurnal Ilmiah Biologi","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136367137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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