Ma Zhidong, Chang Zili, L. Fang, Huo Yanhong, Lin Jianyun, Wu Zhenghua, Zhang Zhongbing
{"title":"巢式PCR扩增布氏田鼠细胞色素C氧化酶峰1基因。","authors":"Ma Zhidong, Chang Zili, L. Fang, Huo Yanhong, Lin Jianyun, Wu Zhenghua, Zhang Zhongbing","doi":"10.3760/CMA.J.ISSN.2095-4255.2015.01.018","DOIUrl":null,"url":null,"abstract":"Objective \nTo determine a method for amplification of cytochrome C oxidase subunit I(CO I) gene of Brandt's vole. \n \n \nMethods \nThe Brandt's Voles were captured in Abagaqi Xilingol League Inner Mongolia, and DNA was extracted from liver tissue. CO I gene was amplified by nested PCR and sequenced afterwards. \n \n \nResults \nA band of 657 bp and 1 132 bp was amplified by internal and external PCR primers, respectively, which were consistent with expected sizes. A total of 12 segments of Brandt's Vole CO I gene sequences were amplified by PCR and verified by sequencing. The sequence number was KF182196 - KF182207 in GenBank. After gene sequence alignment of the 12 CO I gene sequences, it was found that the similarity was 100%, and no base mutation. \n \n \nConclusion \nCO I gene of Brandt's Vole could be amplified by nested PCR without pseudo gene. \n \n \nKey words: \nNested PCR; Brandt's Vole; Cytochrome C oxidase subunit I gene","PeriodicalId":55880,"journal":{"name":"中华地方病学杂志","volume":"57 1","pages":"70-72"},"PeriodicalIF":0.0000,"publicationDate":"2015-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Amplification of cytochrome C oxidase summit I gene of Brandt's Vole by nested PCR.\",\"authors\":\"Ma Zhidong, Chang Zili, L. Fang, Huo Yanhong, Lin Jianyun, Wu Zhenghua, Zhang Zhongbing\",\"doi\":\"10.3760/CMA.J.ISSN.2095-4255.2015.01.018\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective \\nTo determine a method for amplification of cytochrome C oxidase subunit I(CO I) gene of Brandt's vole. \\n \\n \\nMethods \\nThe Brandt's Voles were captured in Abagaqi Xilingol League Inner Mongolia, and DNA was extracted from liver tissue. CO I gene was amplified by nested PCR and sequenced afterwards. \\n \\n \\nResults \\nA band of 657 bp and 1 132 bp was amplified by internal and external PCR primers, respectively, which were consistent with expected sizes. A total of 12 segments of Brandt's Vole CO I gene sequences were amplified by PCR and verified by sequencing. The sequence number was KF182196 - KF182207 in GenBank. After gene sequence alignment of the 12 CO I gene sequences, it was found that the similarity was 100%, and no base mutation. \\n \\n \\nConclusion \\nCO I gene of Brandt's Vole could be amplified by nested PCR without pseudo gene. \\n \\n \\nKey words: \\nNested PCR; Brandt's Vole; Cytochrome C oxidase subunit I gene\",\"PeriodicalId\":55880,\"journal\":{\"name\":\"中华地方病学杂志\",\"volume\":\"57 1\",\"pages\":\"70-72\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-01-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中华地方病学杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3760/CMA.J.ISSN.2095-4255.2015.01.018\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华地方病学杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.2095-4255.2015.01.018","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
Amplification of cytochrome C oxidase summit I gene of Brandt's Vole by nested PCR.
Objective
To determine a method for amplification of cytochrome C oxidase subunit I(CO I) gene of Brandt's vole.
Methods
The Brandt's Voles were captured in Abagaqi Xilingol League Inner Mongolia, and DNA was extracted from liver tissue. CO I gene was amplified by nested PCR and sequenced afterwards.
Results
A band of 657 bp and 1 132 bp was amplified by internal and external PCR primers, respectively, which were consistent with expected sizes. A total of 12 segments of Brandt's Vole CO I gene sequences were amplified by PCR and verified by sequencing. The sequence number was KF182196 - KF182207 in GenBank. After gene sequence alignment of the 12 CO I gene sequences, it was found that the similarity was 100%, and no base mutation.
Conclusion
CO I gene of Brandt's Vole could be amplified by nested PCR without pseudo gene.
Key words:
Nested PCR; Brandt's Vole; Cytochrome C oxidase subunit I gene
期刊介绍：
The Chinese Journal of Endemiology covers predominantly endemic diseases threatening health of the people in the areas affected by the diseases including Keshan disease, Kaschin-Beck Disease, iodine deficiency disorders, endemic fluorosis, endemic arsenism, plague, epidemic hemorrhagic fever, brucellosis, parasite diseases and the diseases related to local natural and socioeconomic conditions; and reports researches in the basic science, etiology, epidemiology, clinical practice, control as well as multidisciplinary studies on the diseases.
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