黄芩苷减弱副猪绿脓杆菌所致腹膜炎的PANX-1/P2X7轴、P2Y6和NLRP3/Caspase-1信号通路

IF 2.1 Q3 MICROBIOLOGY Microbiology Research Pub Date : 2023-08-09 DOI:10.3390/microbiolres14030074
S. Fu, Xinyue Tian, Jingyang Li, Yuzhen Yuan, Xiaoyi Li, Mingxing Ren, Ling Guo, Chun Ye, Bingbing Zong, Yu Liu, Qirong Lu, Y. Qiu
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引用次数: 0

摘要

副猪绿脓杆菌(G.副猪)可引起仔猪腹膜炎。然而,腹膜炎的发病机制尚不清楚。黄芩苷已被证明具有抗炎和抗氧化功能。本研究旨在探讨副猪螺旋体诱导的腹膜炎中PANX-1/P2X7轴和P2Y6信号通路的作用,以及黄芩对副猪螺旋体诱发的PANX-1/P2X7轴和P2Y6通路激活的影响。从具有Glässer病的典型症状,即关节炎、纤维性多浆液炎、出血性肺炎和脑膜炎的市产猪的肺中获得副猪螺旋体血清型5分离株SH0165。35头仔猪随机分为5组,每组7头。各组分为阴性对照组、感染组、黄芩苷25 mg/kg组、黄芩苷50 mg/kg组和黄芩苷100 mg/kg组。结果表明,副猪螺旋体能促进PANX-1/P2X7轴和P2Y6的激活;诱导NLRP3/caspase-1、IL-1β和IL-18的表达;触发PLC/PKC和MLCK/MLC信号激活;降低紧密连接蛋白ZO-1、E-cadherin、Occludins和claudin 1的表达;Western blot检测小鼠腹膜CD14、CD24、CD36、CD47和CD91的表达(p < 0.01;PLC, p < 0.05)。黄芩苷能显著抑制PANX-1/P2X7轴、P2Y6、NLRP3/caspase-1的激活;降低IL-1β和IL-18的表达;减弱PLC/PKC和MLCK/MLC的激活;促进ZO-1、E-cadherin、occludins和claudin 1的表达;并通过Western blot检测副猪弧菌诱导的腹膜CD14、CD24、CD36、CD47和CD91的表达。本研究结果加深了对副猪弧菌引起腹膜炎机制的认识,并为控制副猪弧菌感染提供了一些新的潜在方法。
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Baicalin Attenuated PANX-1/P2X7 Axis, P2Y6, and NLRP3/Caspase-1 Signaling Pathways in Peritonitis Induced by Glaesserella parasuis
Glaesserella parasuis (G. parasuis) can cause peritonitis in piglets. However, the pathogenesis of peritonitis remains unclear. Baicalin has been shown to possess anti-inflammatory and anti-oxidant functions. The aim of this study was to investigate the role of the PANX-1/P2X7 axis and the P2Y6 signaling pathway in peritonitis induced by G. parasuis and the effect of baicain on the PANX-1/P2X7 axis and P2Y6 pathway activation triggered by G. parasuis. A G. parasuis serovar 5 isolate SH0165 strain was obtained from the lungs of commercially produced pigs which had the typical symptoms of Glässer’s disease, namely arthritis, fibrinous polyserositis, hemorrhagic pneumonia, and meningitis. Then, 35 piglets were randomly divided into five groups, each group containing seven piglets. The groups consisted of a negative control group, an infection group, a 25 mg/kg baicalin group, a 50 mg/kg baicalin group, and a 100 mg/kg baicalin group. The results showed that G. parasuis could promote PANX-1/P2X7 axis and P2Y6 activation; induce NLRP3/caspase-1, IL-1β and IL-18 expression; trigger PLC/PKC and MLCK/MLC signaling activation; attenuate the expression of tight junction proteins ZO-1, E-cadherin, Occludins, and claudin 1; and stimulate CD14, CD24, CD36, CD47, and CD91 expression in the peritoneum as measured via Western blot (p < 0.01; PLC, p < 0.05). Baicalin could significantly inhibit PANX-1/P2X7 axis, P2Y6, and NLRP3/caspase-1 activation; reduce IL-1β and IL-18 expression; attenuate PLC/PKC and MLCK/MLC activation; promote ZO-1, E-cadherin, occludins, and claudin 1 expression; and reduce CD14, CD24, CD36, CD47, and CD91 expression in the peritoneum induced by G. parasuis as measured via Western blot. Our results deepen the understanding of the mechanism of peritonitis triggered by G. parasuis and provide some novel potential methods of controlling G. parasuis infection.
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来源期刊
Microbiology Research
Microbiology Research MICROBIOLOGY-
CiteScore
1.90
自引率
6.70%
发文量
62
审稿时长
10 weeks
期刊介绍: Microbiology Research is an international, online-only, open access peer-reviewed journal which publishes original research, review articles, editorials, perspectives, case reports and brief reports to benefit researchers, microbiologists, physicians, veterinarians. Microbiology Research publishes ‘Clinic’ and ‘Research’ papers divided into two different skill and proficiency levels: ‘Junior’ and ‘Professional’. The aim of this four quadrant grid is to encourage younger researchers, physicians and veterinarians to submit their results even if their studies encompass just a limited set of observations or rely on basic statistical approach, yet upholding the customary sound approach of every scientific article.
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