C. Ikechi-Nwogu, B. Odogwu, Maria Adanima Edumasam
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引用次数: 0
摘要
摘要Ikechi-Nwogu CG, Odogwu BA, Edumasam MA。2023. 与尼日利亚哈科特港乔巴市场销售的甜橙腐败有关的丝状真菌的分子特征。农业学报,7:52-56。甜橙采后变质是造成采后损失的主要原因之一。本研究旨在利用分子技术鉴定与尼日利亚河流州乔巴市场甜橙收获后腐败相关的真菌。利用内部转录间隔物4和5分子标记对分离物SO-1和SO-2的DNA进行了鉴定,并利用美国国家生物技术信息中心(NCBI)数据库的Basic Local Alignment Search Tool for Nucleotide (BLASTN) 2.8.0进行了比对。结果表明,分离物SO-1与粗神经孢子菌(Neurospora crassa Shear)和B.O.Dodge的同源性为98.96%,分离物SO-2与黄曲霉(Aspergillus flavus Link)的同源性为99.48%。这些结果表明,草奈瑟菌和黄奈瑟菌是甜橙变质的致病真菌。预计这一结果将提供信息,有助于在收获后处理过程中对这些真菌进行适当的收获后管理,以尽量减少甜橙的腐败,从而减少收获后损失。
Molecular characterization of filamentous fungi associated with spoilage of sweet oranges sold in Choba Market, Port Harcourt, Nigeria
Abstract. Ikechi-Nwogu CG, Odogwu BA, Edumasam MA. 2023. Molecular characterization of filamentous fungi associated with spoilage of sweet oranges sold in Choba Market, Port Harcourt, Nigeria. Asian J Agric 7: 52-56. Post-harvest spoilage of sweet oranges is one of the major causes of post-harvest losses. This study was conducted to identify the fungi associated with post-harvest spoilage of sweet oranges using molecular techniques in Choba Markets, Rivers State, Nigeria. The DNA of the fungal isolate SO-1 and SO-2 were characterized using Internal Transcribed Spacer 4 and 5 molecular markers and aligned by the Basic Local Alignment Search Tool for Nucleotide (BLASTN) 2.8.0 of National Centre for Biotechnology Information (NCBI) database. Based on the sequence similarities, it was observed that isolate SO-1 was 98.96% identical to Neurospora crassa Shear & B.O.Dodge while SO-2 was 99.48% identical to Aspergillus flavus Link. These findings showed that N. crassa and A. flavus are some of the causal fungal pathogens of spoilt sweet oranges. It is anticipated that this result will provide information that will be helpful in the deployment of the appropriate post-harvest management of these fungi during post-harvest handling to minimize the spoilage of sweet oranges and thus reduce post-harvest losses.